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Invitrogen
SARS-COV-2 3CL Protease His-Avi Tag Recombinant Protein
Product Details
RP-88275
Product Specifications
Species
Virus
Expression System
Baculovirus
Amino acid sequence
SARS-CoV-2 3CL protease, amino acids Ser3264-Gln3569 (Accession # YP_009725295.1) with an N-terminal His-tag followed by an Avi-tag
Tag
His-tag, Avi-tag
Molecular weight
37.12 kDa
Class
Recombinant
Type
Protein
Purity
>90% as determined by SDS-PAGE.
Endotoxin concentration
<1.0 EU/µg
Activity
Measured by its ability to cleave a peptide substrate, Dabcyl-KTSAVLQSGFRKME-Edans.The specific activity is >3 pmols/min/ µg
Conjugate
Unconjugated
Form
Lyophilized
Purification
purified
Storage buffer
20mM tris, pH 8, with 10% glycerol, 0.01% mannitol, 300mM NaCl, 5-8% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
It is recommended that sterile water (400 µL) be added to the vial to prepare a stock solution of 0.25 mg/mL.
Concentration is measured by UV.
Target Information
Severe Acute Respiratory Syndrome (SARS), an emerging disease characterized by atypical pneumonia, has been attributed to a novel coronavirus (SARS-CoV). The SARS 3C-like protease (SARS 3CL (pro)) is a cysteine protease engaging in the proteolytic cleavage of the viral precursor polyprotein to a series of functional proteins required for corona virus replication and is considered an attractive target for therapeutics against SARS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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