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Invitrogen
SARS-CoV-2 Spike Protein S1 (B.1.621) His-tag Recombinant Protein
Product Details
RP-88528
Product Specifications
Species
Virus
Expression System
HEK293 cells
Amino acid sequence
SARS-CoV-2 spike S1 (Accession # YP_009724390.1), amino acids Met1-Arg685 with T95I, Y144S, Y145N, R346K, E484K, N501Y, D614G, P681H substitutions and a C-terminal His-tag
Tag
His-tag
Molecular weight
109.57 kDa
Class
Recombinant
Type
Protein
Purity
>85% as determined by SDS-PAGE.
Endotoxin concentration
<1.0 EU/µg
Activity
Immobilized ACE2 Protein, Human, Recombinant (mFc Tag) at 2 µg/mL (100 µL/well) can bind SARS-CoV-2 Spike S1 (T95I, Y144S, Y145N, R346K, E484K, N501Y, D614G, P681H) Protein (His Tag), the EC50 of SARS-CoV-2 Spike S1 (T95I, Y144S, Y145N, R346K, E484K, N501Y, D614G, P681H) Protein (His Tag) is 10-90 ng/mL.
Conjugate
Unconjugated
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4, with 0.01% mannitol, 5-8% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
The mutations were identified in the SARS-CoV-2 varient known as B.1.621
Target Information
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain (Spike Protein). The Spike protein is the main surface antigen of the coronavirus. The glycosylated Spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. CoV diversity is reflected in the variable Spike proteins (S proteins), which have evolved into forms differing in their receptor interactions and their response to various environmental triggers of virus-cell membrane fusion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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