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Invitrogen
SARS-CoV-2 Spike Protein S1/S2 (BA.4) His-tag, trimer Recombinant Protein
Product Details
RP-88549
Product Specifications
Species
Virus
Expression System
HEK293 cells
Amino acid sequence
SARS-CoV-2 spike S1/S2 (Accession # YP_009724390.1) with variant BA.4 mutations expressed with the bacteriophage T4 fibritin and a C-terminal His-tag. Full sequence information available in Product Specfic Information section.
Tag
His-tag
Molecular weight
136.26 kDa
Class
Recombinant
Type
Protein
Purity
>90% as determined by SDS-PAGE. >90% as determined by SEC-HPLC.
Endotoxin concentration
<1.0 EU/µg
Activity
Immobilized human ACE2 protein (mFc tag) at 2 µg/mL (100 µL/well) can bind Recombinant SARS-CoV-2 (BA.4) Spike S1+S2 trimer Protein (ECD, His Tag), the EC50 is 8-32 ng/mL.
Conjugate
Unconjugated
Form
Lyophilized
Concentration
0.25 mg/mL
Purification
purified
Storage buffer
25mM sodium citrate, pH 6, with 5% mannitol, 200mM NaCl, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
It is recommended that sterile water (400 uL) be added to the vial to prepare a stock solution of 0.25 mg/mL.
Concentration is measured by UV.
SARS-CoV-2 spike S1/S2 (Accession # YP_009724390.1) with (LPL24-26, HV69-70) deletions, and F817P, A892P, A899P, A942P, K986P, V987P, T19I, A27S, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K substitutions and furin cleavage site mutations expressed with the bacteriophage T4 fibritin and a C-terminal His-tag.
The mutations were identified in the SARS-CoV-2 varient known as BA.4 or BA.5 or BA.5.2.
Target Information
It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosylated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Coronavirus; COVID; Covid 19; COVID-19; COVID19; SARS; SARS Coronavirus; SARS CoV; SARS CoV-2; SARS CoV2; SARS Spike Protein; SARS-CoV-2
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