Sensitivity compared to fluorescent assay detection with MUNANA substrate

Dilutions of Influenza Type B (ATCC VR-1535) virus culture supernatant (cultured on MDCK cells) were assayed at different temperatures and signal/noise (S/N) ratio calculated using uninfected MDCK cell supernatant (noise). The lower limit of detection (S/N = 2) is at least 30-fold lower with the chemiluminescent NA-Star™ assay, and S/N is approximately 50-fold higher with NA-Star assay. The dynamic range of detection with NA-Star assay with virus samples is three orders of magnitude.

Dilutions of Influenza Type B (ATCC VR-1535) virus culture supernatant (cultured on MDCK cells) were assayed at different temperatures and signal/noise (S/N) ratio calculated using uninfected MDCK cell supernatant (noise). The lower limit of detection (S/N = 2) is at least 30-fold lower with the chemiluminescent NA-<i>Star</i>™ assay, and S/N is approximately 50-fold higher with NA-<i>Star</i> assay. The dynamic range of detection with NA-<i>Star</i> assay with virus samples is three orders of magnitude.

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