Thiol-Reactive Probes—Chapter 2

Introduction to Thiol Modification and Detection—Section 2.1

• Common Applications for Thiol-Reactive Probes
• Reactivity of Thiol Groups
• Iodoacetamides
• Maleimides
• Reversible Thiol-Reactive Reagents
• Reagents for Quantitating Thiols

Thiol-Reactive Probes Excited with Visible Light—Section 2.2

• Alexa Fluor Maleimides
• BODIPY Derivatives
• Fluorescein Derivatives, Including Thiol-Reactive Oregon Green Dyes
• Eosin Maleimide
• Rhodamine Derivatives, Including Thiol-Reactive Texas Red Dyes
• PyMPO Maleimide
• Benzoxadiazole Derivatives, Including NBD Probes
• Lucifer Yellow Iodoacetamide
• TC-FlAsH and TC-ReAsH Detection of Tetracysteine Tagged-Proteins
• Chromophoric Maleimides and Iodoacetamides

Thiol-Reactive Probes Excited with Ultraviolet Light—Section 2.3

• Coumarin Derivatives
• Pacific Orange Maleimide
• Pyrene Derivatives
• Naphthalene Derivatives
• Polar Reagents for Determining Thiol Accessibility
• 1,10-Phenanthroline Iodoacetamide for Preparing Metal-Binding Conjugates

Related Tables

• Thiol-reactive dyes excited with visible light—Table 2.1
• Thiol-reactive dyes excited with ultraviolet light—Table 2.2