Kits containing the supplies for lysing cell samples and measuring beta-galactosidase reporter protein activity within the resulting lysates. Products are designed for use with various cell types and may use a colorimetric assay for detection.
The E. coli lacZ gene, encoding β-D-galactosidase, is extensively used as a reporter gene for detecting the expression of recombinant fusion genes and for monitoring transfection efficiency in mammalian, yeast, and bacterial cells.
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I tested the Mammalian beta-Galactosidase Assay Kit and did not use the Stop Solution. I performed three absorbance readings and the values were different, why is this?