Master mixes, sometimes referred to as 'super mixes' or 'ready mixes' are concentrated, pre-mixed solutions that contain all of the components (precursors, enzymes, etc.) required for Real Time PCR, excluding primers and probes (both For Laboratory Use and For Research Use Only).
Applied Biosystems™ SYBR™ Green Universal Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.
Coupled with user-supplied primer sets and template, PowerUp™ SYBR™ Green Master Mix is designed to amplify targets for accurate gene expression analysis.
TaqMan™ Gene Expression Master Mix has been validated with TaqMan™ Gene Expression Assays and Applied Biosystems™ real-time systems, providing precise quantification for a variety of gene expression-based real-time qPCR applications, including rare transcript detection, gene expression analysis,...
TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).
PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.
Designed for speed, SYBR™ Green Master Mix provides a fast, reliable, and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range, or PCR efficiency.
Applied Biosystems™ TaqMan™ Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (less than 40 minutes).
TaqPath 1-Step RT-qPCR Master Mix, CG, is designed for robust and reproducible one-step pathogen detection and gene expression workflows. The single-tube 4X formulation contains thermostable MMLV reverse transcriptase, dNTPs, UNG, ROX dye, and thermostable Fast DNA polymerase.
The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
Applied Biosystems Absolute Q DNA Digital PCR Master Mix (5X) is optimized for use with the QuantStudio Absolute Q Digital PCR System and Applied Biosystems digital PCR (dPCR) assays in a simple workflow with minimal processing steps.
Particularly useful for RNA virus detection and high throughput gene expression workflows, the mix includes thermostable MMLV reverse transcriptase, dNTPs, UNG, MUSTANG PURPLE™ dye (passive reference), and thermostable Fast DNA polymerase, all in a single tube.
High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences. Unlike some competitive mixes, the MeltDoctor™ HRM Master Mix does not require additional mixing prior to use, and was developed and optimized solely for HRM...
Applied Biosystems TaqMan master mixes have been optimized for use across a wide variety of research and discovery-stage biopharmaceutical applications. Choosing the right master mix depends on your target (RNA or DNA), application, test format, and multiplexing needs.
When using dye-based chemistry for real-time PCR analysis, master mix choice matters. As the pioneers of SYBR Green dye quantification for qPCR, we have evolved our SYBR Green master mix formulations over many years into highly consistent reagents that deliver exceptional sensitivity and specificity...