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PCR Cloning Kits
PCR cloning kits include products and kits needed to conduct polymerase chain reaction (PCR) cloning.
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Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning
Offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proofreading, thermostable DNA polymerases
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing
Efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System
Provides quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector
For fast, efficient cloning and subsequent in vitro transcription/translation
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing
Provides a highly efficient, 5-minute cloning reaction for the direct insertion of proofreading polymerase-amplified, blunt-ended PCR products into a plasmid vector for sequencing
Provides a 5 minute, one-step cloning strategy. Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with pCR4-TOPO Vector, One Shot™ TOP10 Chemically Competent E. coli and PureLink Plasmid Prep Kit enables direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing.
Bac-to-Bac™ C-His TOPO™ Cloning Kit
Bac-to-Bac™ N-His TOPO™ Cloning Kit
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The Thermo Scientific CloneJET PCR Cloning Kit is a versatile, advanced positive selection system for high-efficiency cloning of PCR products. Ligation into the positive selection vector takes only five minutes, yielding more than 99% recombinant clones. All common laboratory E.
Invitrogen TA Cloning technology greatly simplifies traditional restriction and ligation cloning with a one-step cloning strategy that eliminates the need for any enzymatic modifications of the PCR product and not require the use of primers that contain restriction enzyme sites.
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User Guide: InsTAclone PCR Cloning Kit
What concentration is the vector pTZ57R/T supplied at in the InsTAclone PCR Cloning kits?