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DNA Vectors
DNA vectors are molecules used as a vehicle to transfer foreign genetic material into a host cell where it can be replicated and/or expressed. These products include kits for cloning, transfection, and expression of DNA.
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Designed for high-level, constitutive expression in a variety of mammalian cell lines
Destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins
Designed for high-level, constitutive expression in variety of mammalian cell lines
Enables rapid cloning of one or more genes into virtually any protein expression system
Allows high-level, methanol inducible expression of the gene of interest in Pichia, and can be used in Pichia strains including X-33, SMD1168H, and KM71H
For lentiviral-based expression of a target gene in dividing and nondividing mammalian cells
Designed for high-level, constitutive expression in a variety of mammalian cell lines
Features two promoters in single vector for expression of two proteins simultaneously in insect cells when using Bac-to-Bac™ Baculovirus Expression System
Designed for the independent expression of two genes from a single plasmid in mammalian cells
Designed for high-level, constitutive expression in variety of mammalian cell lines
Enables efficent production of recombinant baculovirus for expression testing in insect cells
For native expression of the protein of interest in S. cerevisia
Designed to deliver exceptionally high levels of transgene expression in adherent cells
Destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is entry clone or Ultimate™ RF Clone
Optimize expression and recovery of protein of interest
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Molecular cloning, a key component of the molecular biology workflow, is used to assemble recombinant DNA molecules and to direct their replication. In the molecular cloning workflow, the DNA to be cloned is identified and treated with enzymes to generate DNA fragments.
Lentiviral vectors are a widely used modality for gene modification in cell therapy manufacturing processes. The innovative lentivirus production solutions from Thermo Fisher Scientific are designed to accelerate and streamline the development of gene-modified cell therapies and gene therapies.
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I am interested in using the CRISPR system to modify my gene of interest using HDR instead of NHEJ repair. How can I do this?
Do I need to use the P3000 Reagent when transfecting siRNA? What if I am transfecting shRNA or miRNA in a vector?