Master mixes, sometimes referred to as 'super mixes' or 'ready mixes' are concentrated, pre-mixed solutions that contain all of the components (precursors, enzymes, etc.) required for Real Time PCR, excluding primers and probes (both For Laboratory Use and For Research Use Only).
Coupled with user-supplied primer sets and template, PowerUp™ SYBR™ Green Master Mix is designed to amplify targets for accurate gene expression analysis.
TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).
TaqMan™ Gene Expression Master Mix has been validated with TaqMan™ Gene Expression Assays and Applied Biosystems™ real-time systems, providing precise quantification for a variety of gene expression-based real-time qPCR applications, including rare transcript detection, gene expression analysis,...
Designed for speed, SYBR™ Green Master Mix provides a fast, reliable, and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range, or PCR efficiency.
Particularly useful for RNA virus detection and high throughput gene expression workflows, the mix includes thermostable MMLV reverse transcriptase, dNTPs, UNG, MUSTANG PURPLE™ dye (passive reference), and thermostable Fast DNA polymerase, all in a single tube.
TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications. Applied Biosystems reagents have been validated with TaqMan assays and Applied Biosystems real-time systems to ensure sensitive, accurate, and reliable performance...
The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
Thermo Scientific™ Luminaris Color HiGreen Master Mixes are ready-to-use qPCR master mixes with color and premixed UDG optimized for SYBR Green chemistry using standard cycling protocols.
The master mixes include Thermo Scientific Hot Start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimized PCR buffer. Only the template, primers and probe need to be added. The Hot Start Taq DNA polymerase, in combination with an optimized buffer, ensures PCR specificity and...
Particularly useful for RNA virus detection and high throughput gene expression workflows, the mix includes thermostable MMLV reverse transcriptase, dNTPs, UNG, and thermostable Fast DNA polymerase all in a single tube.
The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
Applied Biosystems TaqMan master mixes have been optimized for use across a wide variety of research and discovery-stage biopharmaceutical applications. Choosing the right master mix depends on your target (RNA or DNA), application, test format, and multiplexing needs.
When using dye-based chemistry for real-time PCR analysis, master mix choice matters. As the pioneers of SYBR Green dye quantification for qPCR, we have evolved our SYBR Green master mix formulations over many years into highly consistent reagents that deliver exceptional sensitivity and specificity...