DNA Vectors | Thermo Fisher Scientific

pcDNA™3.1⁽⁺⁾ Mammalian Expression Vector

Designed for high-level, constitutive expression in a variety of mammalian cell lines

Destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins

pcDNA™6.2-DEST Mammalian Expression Vector

Designed for high-level, constitutive expression in variety of mammalian cell lines

Gateway™ pDONR™221 Vector

Enables rapid cloning of one or more genes into virtually any protein expression system

pLenti6/V5-DEST™ Gateway™ Vector

For lentiviral-based expression of a target gene in dividing and nondividing mammalian cells

pPICZα A, B, & C Pichia Vectors

Allows high-level, methanol inducible expression of the gene of interest in Pichia, and can be used in Pichia strains including X-33, SMD1168H, and KM71H

pcDNA™3.1^(-) Mammalian Expression Vector

Designed for high-level, constitutive expression in a variety of mammalian cell lines

pYES2 Yeast Expression Vector

For native expression of the protein of interest in S. cerevisia

pFastBac™ Dual Expression Vector

Features two promoters in single vector for expression of two proteins simultaneously in insect cells when using Bac-to-Bac™ Baculovirus Expression System

Gateway™ pDEST™24 Vector

Destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is entry clone or Ultimate™ RF Clone

pBudCE4.1 Mammalian Expression Vector

Designed for the independent expression of two genes from a single plasmid in mammalian cells

Bac-to-Bac™ Baculovirus Expression System

Enables efficent production of recombinant baculovirus for expression testing in insect cells

X-33, Pichia pastoris Yeast Strain

Optimize expression and recovery of protein of interest

pYES2.1 TOPO™ TA Yeast Expression Kit

Offers direct cloning of Taq-amplified PCR product into a Saccharomyces cerevisiae expression vector

pcDNA™3.1/V5-His TOPO™ TA Expression Kit

Provides efficient, 5-minute, one-step cloning strategy for direct insertion of Taq polymerase-amplified PCR products into plasmid vector

Thermo Scientific Molecular Cloning

Molecular cloning, a key component of the molecular biology workflow, is used to assemble recombinant DNA molecules and to direct their replication. In the molecular cloning workflow, the DNA to be cloned is identified and treated with enzymes to generate DNA fragments.

Lentiviral Vector Production Workflow

Lentiviral vectors are a widely used modality for gene modification in cell therapy manufacturing processes. The innovative lentivirus production solutions from Thermo Fisher Scientific are designed to accelerate and streamline the development of gene-modified cell therapies and gene therapies.

Product FAQ

I am interested in using the CRISPR system to modify my gene of interest using HDR instead of NHEJ repair. How can I do this?

Product FAQ

Do I need to use the P3000 Reagent when transfecting siRNA? What if I am transfecting shRNA or miRNA in a vector?