Modifying Enzymes

Purified from bovine pancreas

Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic, or hydrophobic amino acids. The Proteinase K is classified as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.

Catalyze the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with the Thermo Scientific™ T4 DNA Ligase. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids.

Contains enzymes and buffer in a single mix to enable convenient ten-microliter reaction set-up with fewer pipetting steps.

Ideal tool for clearing residual DNA

Catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3' hydroxyl and 5' phosphate termini

Gateway™ LR Clonase™ enzyme mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor) and Xis (Excisionase) enzymes that catalyze the in vitro recombination between an Entry clone (containing a gene of interest flanked by attL sites) and a Destination vector (containing attR...

Exonuclease III (ExoIII) exhibits four catalytic activities (see Reference1). The 3'≥5' exodeoxyribonuclease activity of ExoIII is specific for double-stranded DNA. ExoIII degrades dsDNA from blunt ends, 5'-overhangs or nicks, releases 5'-mononucleotides from the 3'-ends of DNA strands and produces...

Catalyzes in vitro recombination of PCR products to generate entry clones

Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.

Invitrogen TrueCut Cas9 Protein v2 is a next-generation CRISPR Cas9 protein engineered to deliver maximum editing efficiency.

A high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency. TrueCut™ HiFi Cas9 Protein is ideal for applications that are sensitive to off-target events and when more precise editing is required.

SuperScript™ IV VILO™ Master Mix is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.

A high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency. TrueCut™ HiFi Cas9 Protein is ideal for applications that are sensitive to off-target events and when more precise editing is required.