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Substrates
A variety of substrates in various quantities designed for biological and chemical analyses, the initiation of chemical reactions, culturing procedures, and use as a matrix for organisms to live in or on. Products are suited for specialty applications.
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Novex™ AP Chemiluminescent Substrate (CDP-Star™)
Novex™ AP Chromogenic Substrate (BCIP/ NBT)
CCF2-FA (free acid)
CCF4-AM
This CDP-Star™ chemiluminescent substrate for alkaline phosphatase let you detect alkaline phosphatase and alkaline phosphatase-labeled molecules with unparalleled sensitivity, speed, and ease.
CCF2-AM
MUP is a fluorescent substrate for alkaline phosphatases
This concentrated CSPD™ substrate is a chemiluminescent alkaline phosphatase substrate for protein or nucleic acid blotting on nitrocellulose membranes. This substrate can also be used in solution-based assays.
Widely used chromogenic substrate for β-galactosidase
Cell permeant
A truncated version (residues 1-179) of c-Jun has been expressed as a fusion with GFP (Green Fluorescent Protein) to act as a substrate for the JNK family of kinases.
4E-BP1 expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for FRAP1 (mTor).
Vivid™ BOMF Substrate
Continuously assay acid phosphatases at low pH
Perform a simple colorimetric method for lysing cultured mammalian cells and measuring beta-Gal activity using Thermo Scientific™ Mammalian β-Galactosidase Assay Kit.
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Bluo-gal and X-gal are commonly used substrates for measuring enzymatic activity of beta-galactosidase in bacteria. Life Technologies offers these enzyme substrates in several sizes for added convenience.
Enzyme-linked immunoassays (ELISAs) are used to quantitate specific proteins in solution. The detection enzyme can be introduced into the assay in a number of ways ( Figure 1 ). Horseradish peroxidase (HRP) and alkaline phosphatase (AP) are the most commonly used enzyme labels, mainly due to the...
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Can I use SuperSignal West Femto Maximum Sensitivity Substrate with the identical protocol (i.e., same dilutions) I used with the SuperSignal West Pico Chemiluminescent Substrate?
Can I use SuperSignal West Dura Extended Duration Substrate with the identical protocol (i.e., same dilutions) I used with the SuperSignal West Pico Chemiluminescent Substrate?