Thermo Scientific EpiJET Bisulfite Conversion Kit is designed for simple and reliable bisulfite conversion of DNA for methylation analysis. In the bisulfite reaction all unmethylated cytosines are deaminated and converted to uracils, while methylated cytosines remain unchanged.
Cytochalasin D is a cell permeable fungal toxin that binds to the barbed end of actin filaments inhibiting both the association and dissociation of subunits.
Deanimate unmethylated cytosines and convert them to uracils. Thermo Scientific™ EpiJET™ Modification Solution II is a component of the EpiJET™ Bisulfate Conversion Kit (K1461) and may be purchased separately when additional supply is needed for more reactions. Supplied in 0.35mL aliquots.
Deanimate unmethylated cytosines and convert them to uracils. Thermo Scientific™ EpiJET™ Modification Solution I is a component of the EpiJET™ Bisulfate Conversion Kit (K1461) and may be purchased separately when additional supply is needed for more reactions. Supplied in 1.2mL aliquots.
Wash bound DNA to remove any contaminants from the modified DNA. Thermo Scientific™ EpiJET™ Wash Buffer (concentrated) is a component of the EpiJET™ Bisulfate Conversion Kit (K1461) and may be purchased separately when additional supply is needed. Provided in 9mL aliquots.
Thermo Fisher Scientific is a global leader in the manufacture and supply of nucleic acid modifying enzymes and polymerases. We offer a combination of proprietary as well as generic off-the-shelf enzymes, and can also custom-manufacture on your behalf.
Protease and phosphatase inhibitors are essential components in biochemical research and protein studies as they safeguard the structural integrity of proteins during the extraction and analysis processes.
I am processing fecal samples with the MagMAX Total Nucleic Acid Kit (Cat. No. AM1840) and get very low A260/A230 ratios. Do you have any recommendations for removing some of these contaminants?