Gel Electrophoresis Buffers and Diluents

NuPAGE Sample Reducing Agent (10X) is used to reduce protein samples for protein gel electrophoresis. It contains 500 mM dithiothreitol (DTT) for a 10X concentration in a stabilized liquid form. See all available buffers and reagents available for SDS-PAGE

NuPAGE LDS Sample Buffer (4X) is used to prepare protein samples for denaturing gel electrophoresis with Bis-Tris or Tris-Acetate gels. It contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.

The NativePAGE™ Running Buffer Kit contains the NativePAGE™ Running Buffer (20X), which is used to make the NativePAGE™ Cathode and Anode Running Buffers for use with an XCell SureLock® Mini Cell when running NativePAGE™ Gels.

IEF Cathode Buffer pH 3-10 (10X) is optimized pre-mixed IEF cathode buffer for using with Novex™ pH 3-10 IEF gels. IEF is a sensitive technique which is affected by many factors. The optimized pre-mixed IEF buffers reduce variability and ensure consistent results.

Novex™ Bolt™ buffers and sample reducing agents are optimized for use with Bolt Bis-Tris Plus gels, and are available in a variety of formats.

NuPAGE Transfer Buffer (20X) is used to transfer proteins from NuPAGE Bis-Tris and NuPAGE Tris-Acetate gels to membranes for western blotting. It maintains the neutral pH environment established during electrophoresis.

BlueJuice Gel Loading Buffer (10X) is designed for easy loading and tracking of DNA samples in agarose or native polyacrylamide gels. Visualization of DNA bands will not be obscured by the tracking dyes because they run outside the limits of most DNA samples.

For protein sample preparation to be used in the Laemmli SDS-PAGE system. Laemmli SDS sample buffer is used as an electrophoretic dye for denaturation of proteins and monitoring the front of running gel. Further, it is used for polyacrylamide protein gel analysis.

6X DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains two different dyes (bromophenol blue and xylene cyanol FF) for visual tracking of DNA migration during electrophoresis.

NativePAGE Running Buffer (20X) is used to make the NativePAGE Cathode and Anode running buffers for use with a Mini Gel tank or an XCell SureLock Mini Cell when running NativePAGE gels. This buffer is critical for maintaining a near-neutral pH environment during electrophoresis, which is vital in...

Laemmli SDS sample buffer is used for protein sample preparation to be used in the Laemmli SDS-PAGE system, The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size.

Pierce Endotoxin-Free MgCl2 is suitable for the preparation of samples for endotoxin detection (LAL) assay. As a diluent, it will eliminate the inhibitory chelation effects of EDTA. It contains <0.005 EU/mL endotoxin.

ZOOM® Carrier Ampholytes are small, soluble molecules with both positive and negative charge groups. They sort based on their isoelectric points in an electric field, thereby establishing a pH gradient in solution or contributing to the gradient when used with IPG strips.

ZOOM® Carrier Ampholytes are small, soluble molecules with both positive and negative charge groups. They sort based on their isoelectric points in an electric field, thereby establishing a pH gradient in solution or contributing to the gradient when used with IPG strips.

ZOOM® Carrier Ampholytes are small, soluble molecules with both positive and negative charge groups. They sort based on their isoelectric points in an electric field, thereby establishing a pH gradient in solution or contributing to the gradient when used with IPG strips.