Competent Cells

Thermo Scientific DH5α Competent Cells are high efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar...

One Shot TOP10 Chemically Competent E. coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 109 cfu/μg plasmid DNA. These cells allow stable replication of high-copy number plasmids and are the same competent cells that come...

MAX Efficiency™ DH5α Competent Cells are a well-known, versatile strain that is used in many everyday cloning applications. Prepared by a proprietary modification of the Hanahan method, these cells are suitable for high efficiency transformations in applications where low amount of DNA is used or to...

Subcloning Efficiency DH5α Competent Cells are a versatile, chemically competent strain for cloning that provides a transformation efficiency of >1 x 106 cfu/μg plasmid DNA. Subcloning Efficiency DH5α Competent Cells are an economical solution for routine subcloning procedures or any general...

One Shot BL21(DE3)pLysS Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET. The pLysS plasmid carried by the BL21(DE3) pLysS strain produces T7 lysozyme, an inhibitor of T7 RNA polymerase, to reduce basal level...

One Shot BL21 Star (DE3) Chemically Competent E. coli are designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7-promoter-based expression systems such as Champion pET vectors.

FreeStyle™ 293 Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F cells in suspension without adaptation. The medium is complete and ready-to-use, with no supplementation required.

Gibco® CD Hybridoma Medium is an animal origin-free, chemically-defined, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® CD Hybridoma Medium features: • Ability to support suspension and stationary hybridomas and myelomas...

FreeStyle™ CHO Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transient transfection of CHO-S cells in suspension without adaptation. This medium requires supplementation with 8 mM glutamine before use.

The Tali™ Viability Kit - Dead Cell Red is a ready-to-use solution of propidium iodide (PI) that has been validated for use with the Tali™ Image-Based Cytometer. PI is a live cell-impermeant fluorogenic DNA-binding dye that has been extensively used to identify necrotic cells.

The Tali™ Cell Cycle Kit includes a ready-to-use reagent that, when used with the Tali™ Image-Based Cytometer, provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.

This product contains three tubes of beads which are used to properly calibrate and align the Tali™ Image-Based Cytometer. Contents: • Tali™ Alignment Beads, • Tali™ Green Calibration Beads • Tali™ Red Calibration Beads The calibration function of the Tali™ Image-Based Cytometer recalculates the...

MAX Efficiency DH5αF´IQ Competent Cells are high transformation efficiency chemically competent cells prepared by a patented modification of the procedure of Hanahan. This strain is a derivative of the DH5α strain, sharing the same benefits.

The One Shot OmniMAX 2 T1R E. coli strain is an improved chemically competent cell line that can achieve extremely high transformation efficiency of >5 x 109 transformants/μg pUC19 DNA. It offers the highest transformation efficiency of any chemically competent cells in a One Shot format.

Expi293 Met (–) Expression Medium is a cGMP-manufactured, chemically defined, serum-free, protein-free, animal origin-free, methionine-free medium for growth and transfection of Expi293F cells. The medium is part of the Expi293 platform for rapid, high-yield transient protein expression from...