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Competent Cells
Explore our comprehensive selection of competent cells for various molecular biology applications. This collection includes chemically and electrocompetent E. coli strains designed for high-efficiency cloning, plasmid DNA propagation, and protein expression. Choose from popular strains like DH5α, TOP10, BL21(DE3), and OmniMAX 2 T1R, suitable for a wide range of transformation needs. Our competent cells offer different transformation efficiencies and formats to suit your specific workflow and budget requirements. Learn more about competent cells.
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One Shot Stbl3 Chemically Competent E. coli are designed for cloning direct repeats found in lentiviral expression vectors as these cells reduce the frequency of homologous recombination of direct repeats found in lentiviral vectors (ViraPower Lentiviral Expression kits) and other retroviral vectors...
One Shot TOP10 Chemically Competent E. coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 109 cfu/μg plasmid DNA.
MAX Efficiency™ DH5α Competent Cells are a well-known, versatile strain that is used in many everyday cloning applications. Prepared by a proprietary modification of the Hanahan method, these cells are suitable for high efficiency transformations in applications where low amount of DNA is used or to...
One Shot BL21 Star (DE3) Chemically Competent E. coli are designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7-promoter-based expression systems such as Champion pET vectors.
Subcloning Efficiency DH5α Competent Cells are a versatile, chemically competent strain for cloning that provides a transformation efficiency of >1 x 106 cfu/μg plasmid DNA.
ElectroMAX Stbl4 Competent Cells are specifically designed for cloning unstable inserts. This strain is a derivative of Stbl2 cells (engineered from JM109/J5 strain).
One Shot ccdB Survival 2 T1R Competent Cells are suitable for the propagation of plasmids containing the ccdB gene and are designed for use with the Gateway Vector Conversion System and for propagating Gateway destination, donor, and supercoiled entry vectors.
MegaX DH10B T1R Electrocomp Cells are the highest-efficiency electrocompetent cells available. Improved production processes provide a transformation efficiency reaching >3 x 1010 cfu/μg of pUC DNA.
ElectroMAX DH10B Cells are electrocompetent E. coli cells offering the highest transformation efficiencies of >1 x 1010 cfu/μg plasmid DNA.
MAX Efficiency DH10B Competent Cells are highly efficient E. coli suitable for a variety of molecular biology applications. This strain is one of the most commonly used strains for everyday applications due to key strain features such as high transformation efficiency.
Expi293 Met (–) Expression Medium is a cGMP-manufactured, chemically defined, serum-free, protein-free, animal origin-free, methionine-free medium for growth and transfection of Expi293F cells.
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Thermo Scientific DH10B, DH5α, and BL21(DE3) competent cells are produced with stringent quality control to enable reliable, consistent performance in cloning workflows. Thermo Scientific DH10B competent cells are also included in the Thermo Scientific CloneJET PCR Cloning Kit and in the Thermo...
TOP10 cells are the most popular E. coli strain engineered for high transformation efficiency (>1 x 10 9 cfu/µg). They are suitable for cloning supercoiled DNA and allow stable replication of high-copy number plasmids.
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What is the formulation of the SOC medium that is provided with competent cells?
Flyer: Confidently Competent - Thermo Scientific Competent Cells