PCR Specific Buffers

PCR Specific Buffers

Buffers optimized for specific PCR procedures. Products are available in various volumes and concentrations and for different PCR-related processes.
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Taq DNA Polymerase PCR Buffer is a 10X buffer [200 mM Tris HCl (pH 8.4), 500 mM KCl] supplied with 1 mL of 50 mM MgCl2.
Thermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA polymerases (both recombinant and native). They are available in different compositions and also without detergents.
PCRX Enhancer System is an optimized buffer and cosolvent system designed for use in conjunction with DNA polymerases to optimize PCR of problematic and/or GC-rich templates. 10X PCRX Enhancer Solution with 10X PCRX Amplification Buffer offers higher primer specificity, broader magnesium...
Thermo Scientific PCR reagents meet high quality standards to generate accurate results from even the most challenging molecular biology applications.
Applied Biosystems™ GeneAmp™ 10X PCR Buffer I is optimized for use with AmpliTaq™ DNA Polymerase, resulting in robust PCR amplification.
Thermo Scientific DreamTaq Buffer (10X), available in colorless and green formats, is specifically optimized for PCR using DreamTaq DNA Polymerase and DreamTaq Hot Start DNA Polymerase. It contains both KCl and (NH4)2SO4 to provide high specificity of primer annealing at a wide range of MgCl2...
The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification.
The Invitrogen Buffer Kit provides high quality, RNase free buffers for critical RNA analysis procedures.
Invitrogen™ SuperFi™ Buffer is a 5X buffer solution optimized for use with Invitrogen™ Platinum™ SuperFi DNA Polymerase. The buffer contains 7.5 mM MgCl2 which provides 1.5 mM MgCl2 in the final reaction.
SuperScript IV RT Reaction Buffer is included with, and designed for use with, SuperScript IV Reverse Transcriptase.
SSC solution is commonly used in nucleic acid hybridization techniques at concentrations ranging from 0.1X to 20X depending on the application. It is used to facilitate transfer of nucleic acids to membranes in Southern and Northern blotting, dot blotting, and colony and plaque lifts.
M-MLV Reverse Transcriptase Buffer is for use with Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT).
This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to...
For the polyadenylation of in vitro transcribed RNA to enhance translation initiation efficiency.
The Invitrogen™ QuantumRNA™ 18S Internal Standard with Competimer™ technology uses specially modified Competimers of the same sequence as the normal 18S primers that cannot be extended.
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PCR (Polymerase Chain Reaction) is used in a wide variety of applications including cloning, gene expression analysis, genotyping, sequencing, and mutagenesis. The Thermo Scientific PCR portfolio has PCR and qPCR consumables for every successful PCR run: thermal cyclers, plates, tubes, sealing...
PCR, or DNA polymerase chain reaction, is a widely used laboratory technique in molecular biology to make multiple copies of a specific DNA sequence up to 5 kb in size. Taq polymerase, either native or recombinant, is used for routine PCR experiments.
Documents & Support (101)
How many freeze cycles do you recommend for Applied Biosystems, GeneAmp 10X PCR Buffer I (Cat. No. 4379876)?
With Platinum II Taq Hot-Start DNA Polymerase and Platinum II PCR buffer, how is it possible to use an annealing temperature of 60 degrees C for any primer pair?