Dissociation Reagents

Dissociation Reagents

Browse through our selection of dissociation reagents, essential compounds that facilitate the detachment of cells from surface substrates. These products include trypsin, collagenase, cell dissociation buffers, and enzyme-free solutions. Dissociation reagents are available in various formulations, including liquid, frozen, and powder forms, with options for sterile-filtered and non-sterile preparations. They are used in cell culture applications to separate adherent cells from culture vessels or to dissociate tissue samples into single-cell suspensions. Common types include trypsin-EDTA solutions, recombinant enzymes like TrypLE reagent, and gentle alternatives such as Accutase. These reagents are crucial for cell passaging, harvesting, and preparing samples for downstream analysis in research and bioproduction settings. Learn more about cell dissociation.

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Animal origin-free, recombinant enzyme
Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas.
Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas
Animal origin-free
Gibco TrypLE Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. We offer a variety of TrypLE formulations for a range of cell culture applications.
An EDTA solution for use as a gentle non-enzymatic cell dissociation reagent
Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas
Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas
StemPro Accutase Cell Dissociation Reagent is a cell detachment solution of proteolytic and collagenolytic enzymes.
Unlike porcine trypsin, free of animal-derived components
Invitrogen™ Accutase Enzyme Cell Detachment Medium performs exceptionally well in detaching cells for analysis of cell surface markers and virus growth assay.
TrypLE™ Select Enzyme (10X), no phenol red
Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Glyc-Pro, which is found with high frequency in collagen
Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Gly-Pro, which is found with high frequency in collagen
Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Glyc-Pro, which is found with high frequency in collagen
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To acquire a suitable number of cells for research, a group of cells must first be disaggregated from a larger culture. Enter: cell dissociation. Gibco trypsin and alternative cell dissociation products are ideal for use with tissues and cell monolayers.
StemPro Accutase is a marine-origin enzyme with proteolytic and collagenolytic activity for the detachment of primary and stem cell lines and tissues. Order now, Efficient - gently detach adherent cells within minutes Convenient and cost effective - stable when frozen, refrigerated, and at room...
Documents & Support (250)
How long can I store StemPro Accutase Cell Dissociation Reagent at 2 to 8 degrees C?
With StemPro Accutase Cell Dissociation Reagent, do I need to stop the dissociation reaction with serum?
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