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Chemical Transformation Kits
Chemical transformation kits and reagents are designed to perform chemical transformations in microorganisms such as yeast, cells, bacteria, and more. These products include secretion signal sets, bacterial transformation kits, bacterial expression kits, and secretion optimization kits.
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Employ a novel method for rapid preparation of chemically-competent E. coli cells from overnight bacterial cultures or bacterial colonies with the Thermo Scientific TransformAid™ Bacterial Transformation Kit.
The PichiaPink™ Secretion Signal Set is a set of 8 DNA fragments encoding secretion signal sequences. The PichiaPink™ Secretion Signal Set is designed to be easily ligated in front of your gene.
The Original Pichia Expression Kit is designed for high-level expression of recombinant protein in the yeast Pichia pastoris.
The EasySelect™ Pichia Expression Kit provides all needed components for protein production in the yeast Pichia pastoris.
The Multi-Copy Pichia Expression Kit contains the pPIC3.5K, pPIC9K, and pAO815 vectors for production and selection of Pichia strains that contain more than one copy of the gene of interest.
The PichiaPink™ Secretion Optimization Kit is a new recombinant protein expression system based on the yeast Pichia pastoris.
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Bacterial transformation is a primary technique in molecular cloning to produce multiple copies of a recombinant DNA molecule. Bacterial transformation is a process in which foreign DNA is introduced into bacterial cells, resulting in the acquisition of new genetic traits.
You have worked hard to clone your DNA fragment of interest—you have performed restriction digestion, fragment preparation and purification of the desired insert, vector and insert ligation, bacterial transformation, and finally plating of transformed colonies.
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When should I use the Pichia EasyComp Transformation Kit as opposed to electroporation?
Why is Beta-mercaptoethanol (BME) no longer included with the One Shot Chemically Competent E. coli kits? What was the purpose of the BME during E. coli transformation?