Enzymes and Inhibitors

Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.

Catalyze the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with the Thermo Scientific™ T4 DNA Ligase. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids.

Purified from bovine pancreas

Rigorously tested for contaminating nonspecific endonuclease, exonuclease, and protease activity

Excises uracil from dU-containing DNA by cleaving the N-glycosidic bond between the uracil base and the sugar backbone

Double-stranded RNA (dsRNA) specific endoribonuclease

FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in a single buffer.

Conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions.

Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.

Lambda DNA digested with HindIII, 0.7% agarose, 6 cleavage sites FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

Lambda DNA digested with TaiI, 1.4% agarose, 143 cleavage sites FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

Thermo Scientific TheraPure GMP RNase Inhibitor is manufactured to ICH Q7 GMP principles and supported by a comprehensive documentation package.

Lambda DNA, 0.7% agarose, 2 cleavage sites FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

Lambda DNA digested with NcoI, 0.7% agarose, 4 cleavage sites FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins.