Modifying Enzymes

Gateway™ LR Clonase™ enzyme mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor) and Xis (Excisionase) enzymes that catalyze the in vitro recombination between an Entry clone (containing a gene of interest flanked by attL sites) and a Destination vector (containing attR...

Invitrogen™ ezDNase™ Enzyme is a recombinant double-strand-specific DNase for the fast removal of contaminating genomic DNA from RNA preparations. It cleaves phosphodiester bonds in double-stranded DNA to yield 2–8 bp oligonucleotides with 5’-phosphate and 3’-hydroxyl termini.

The TURBO DNA-free™ Kit contains reagents for the efficient, complete digestion of DNA along with the removal of the enzyme and divalent cations post-digestion. Note: if you would like to purchase the enzyme alone, without the inactivation and cation removal reagents, please see TURBO™ DNAase.

Gateway™ LR Clonase™ II enzyme mix catalyzes the in vitro recombination between an entry clone (containing a gene of interest flanked by attL sites) and a destination vector (containing attR sites) to generate an expression clone.

Catalyze the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with the Thermo Scientific™ T4 DNA Ligase. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids.

Gateway® LR Clonase® enzyme mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor) and Xis (Excisionase) enzymes that catalyze the in vitro recombination between an Entry clone (containing a gene of interest flanked by attL sites) and a Destination vector (containing attR...

The BaculoDirect™ Baculovirus Expression System is a powerful and versatile eukaryotic system for high-level protein expression in insect cells. The combination of Gateway® Technology with baculovirus expression makes the BaculoDirect™ System the fastest and easiest method for production of...

Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.

Catalyze the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with the Thermo Scientific™ T4 DNA Ligase. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids.

Catalyze the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA with the Thermo Scientific™ T4 DNA Ligase. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids.

Bacterial Alkaline Phosphatase (BAP) removes 3´ and 5´ phosphates from DNA and RNA. BAP is active at 65°C for at least 1 h and is inactivated by phenol extraction. Applications: Dephosphorylation of 5´-phosphorylated termini of vector DNA to prevent self-ligation.

Thermo Scientific™ E. coli DNA Ligase is an NAD-dependent DNA ligase. It catalizes formation of phosphodiester bonds between 5`-phospate and 3`-hydroxyl termini in double-stranded DNA. The enzyme is not active on blunt-ended DNA.

Terminal Deoxynucleotidyl Transferase (TdT), a template-independent DNA polymerase, catalyzes the repetitive addition of deoxyribonucleotides to the 3'-OH of oligodeoxyribonucleotides and single-stranded and double-stranded DNA.

Thermo Scientific TheraPure GMP* DNase I, RNase-free, is manufactured to ICH Q7 GMP principles and supported by a comprehensive documentation package. It is designed for DNA template removal during the development and manufacturing of mRNA therapeutics and vaccines.

DNA-free™ DNase treatment and removal reagents are designed for the removal of contaminating DNA from RNA samples and for the removal of DNase after treatment. Features of this reagent set include: • Safely eliminate DNA contamination from RNA samples • No organic extraction or heat inactivation...