Protein Mass Spectrometry Sample Prep

EasyPep Mini MS Sample Prep Kits enable efficient and reproducible processing of cultured mammalian cells and tissues for proteomic MS analysis. The kit contains pre-formulated buffers, MS-grade enzyme mix, peptide clean-up columns, and an optimized protocol to generate MS-compatible peptide samples...

Protein characterization, identification, and quantification by MS begins with efficient, reproducible protein digestion. Sequence specific proteases are utilized to cleave proteins into smaller fragments or peptides. Trypsin is the protease of choice for protein digestion.

Thermo Scientific™ Pierce ESI Negative Ion Calibration Solution is a mixture of highly purified ionizable molecules designed for negative mode calibration of Thermo Scientific™ LTQ series mass spectrometer instruments.

Concentrations of albumin and IgG can make up more than 70% of total serum proteins and often mask lower quantity proteins during protein-profiling experiments. Optimize your serum sample prep by removing albumin or IgG quickly and conveniently.

Thermo Scientific™ Pierce Peptide Retention Time Calibration Mixture enables reversed phase liquid chromatography (LC) and mass spectrometry (LC-MS) users to optimize and confirm correct operation of their equipment.

Thermo Scientific Pierce Protein Precipitation Plates provide an easy, 96-well format for simple, rapid and automatable protein precipitation and filtration (purification) from plasma, serum and other biological fluid samples.

The Thermo Scientific™ Pierce™ Triple Quadrupole Calibration Solution, Extended Mass Range, is a mixture of 14 highly pure, ionizable components (mass range: 69 m/z to 2800m/z) designed for both positive and negative ionization calibration of Thermo Scientific Triple Stage Quadrupole instruments.

Accurate identification and analysis of proteins is a central component of proteomic strategies for studying cellular functions and processes. Using mass spectrometry, proteins and relevant post-translational modifications can be accurately identified, but the method is highly dependent on the...

Proteomic sample preparation for MS analysis is complex, resulting in challenges with reproducibility and peptide quality. For MS sample preparation methods that utilize magnetic beads, a major challenge is the compatibility of the beads with the harsh conditions required for sample processing.

Mass spectrometry (MS) is a key tool for identifying sites of protein phosphorylation and quantifying phosphorylation changes. However, MS analysis of protein phosphorylation is challenging due to the low stoichiometry, high hydrophilicity, poor ionization, and incomplete fragmentation of...

Pierce colorimetric or fluorescent quantitative peptide assays are easy mix-and-read microplate assays with stable signals for accurate and robust measurement of peptide digest samples. A high-quality peptide digest reference standard is provided as a stand-alone, or in the kits for use in the...

Immunoprecipitation is often necessary for the detection and quantitation of low-abundance proteins or rare signaling events. Mass spectrometry analysis relies on two methods for the detection of low-abundance proteins: single reaction monitoring (SRM) and parallel reaction monitoring (PRM), which...

Thermo Scientific™ MALDI Matrices are highly purified, recrystallized reagents packaged in convenient single-use tubes sufficient for analyzing up to 96 samples by matrix assisted laser desorption ionization.

Use the Thermo Scientific™ Pierce™ HeLa Protein Digest Standard, a highly validated mammalian protein digest, as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.

Matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) mass spectrometry (MS) provide high sensitivity and mass accuracy; however, interfering molecules from the sample can result in reduced sensitivity and poor-quality spectra.