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Protein Mass Spectrometry Sample Prep
Browse through our comprehensive selection of protein mass spectrometry sample prep products. This category includes a wide range of supplies, reagents, and kits designed for fractionation, purification, isolation, clean-up, and recovery of total or post-translational modification (PTM)-specific classes of processed peptide fragments for proteomics analysis by mass spectrometry (MS). The product lineup encompasses calibration solutions, chromatography cartridges, columns, digestion kits, intact protein standard mixes, peptide clean-up tips, phosphopeptide enrichment kits, in-gel tryptic digestion kits, magnetic bead sample prep kits, protein digest standards, quantitative peptide assay kits, protease mixes, membrane protein standards, immunoprecipitation-MS sample prep kits, and abundant protein depletion resins. These tools are essential for preparing samples for MS analysis, enabling efficient and reproducible processing of various biological materials such as cell lysates, tissues, plasma, and protein samples. Learn more about mass spectrometry sample preparation products and processes.
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EasyPep Mini MS Sample Prep Kits enable efficient and reproducible processing of cultured mammalian cells and tissues for proteomic MS analysis. The kit contains pre-formulated buffers, MS-grade enzyme mix, peptide clean-up columns, and an optimized protocol to generate MS-compatible peptide samples...
Thermo Scientific Pierce Peptide Desalting Spin Columns are ready-to-use centrifuge spin columns that enable efficient desalting of peptide samples following enzymatic digestion. The polymer-based hydrophobic resin contained in each column provides excellent binding and recovery characteristics for...
Analysis of human fluids is often complicated by the presence of high concentrations of serum proteins, including albumin and IgG that can make up more than 70% of total serum protein. Removal of albumin, immunoglobulins, and other abundant proteins is essential for the study of low-abundance...
Protein characterization, identification, and quantification by MS begins with efficient, reproducible protein digestion. Sequence specific proteases are utilized to cleave proteins into smaller fragments or peptides. Trypsin is the protease of choice for protein digestion.
Proteomic sample preparation for MS analysis is complex, resulting in challenges with reproducibility and peptide quality. For MS sample preparation methods that utilize magnetic beads, a major challenge is the compatibility of the beads with the harsh conditions required for sample processing.
Calibrate and maintain quality control for mass spectrometry (MS) analysis of proteomic samples using the Thermo Scientific™ Pierce™ 6 Protein Digest Standard, Equimolar, LC-MS Grade, a highly validated equimolar protein digest mixture.
Pierce colorimetric or fluorescent quantitative peptide assays are easy mix-and-read microplate assays with stable signals for accurate and robust measurement of peptide digest samples. A high-quality peptide digest reference standard is provided as a stand-alone, or in the kits for use in the...
Thermo Scientific Pierce Protein Precipitation Plates provide an easy, 96-well format for simple, rapid and automatable protein precipitation and filtration (purification) from plasma, serum and other biological fluid samples.
Concentrations of albumin and IgG can make up more than 70% of total serum proteins and often mask lower quantity proteins during protein-profiling experiments. Optimize your serum sample prep by removing albumin or IgG quickly and conveniently.
Immunoprecipitation is often necessary for the detection and quantitation of low-abundance proteins or rare signaling events. Mass spectrometry analysis relies on two methods for the detection of low-abundance proteins: single reaction monitoring (SRM) and parallel reaction monitoring (PRM), which...
Thermo Scientific™ Pierce Peptide Retention Time Calibration Mixture enables reversed phase liquid chromatography (LC) and mass spectrometry (LC-MS) users to optimize and confirm correct operation of their equipment.
Matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) mass spectrometry (MS) provide high sensitivity and mass accuracy; however, interfering molecules from the sample can result in reduced sensitivity and poor-quality spectra.
Mass spectrometry (MS) is a key tool for identifying sites of protein phosphorylation and quantifying phosphorylation changes. However, MS analysis of protein phosphorylation is challenging due to the low stoichiometry, high hydrophilicity, poor ionization, and incomplete fragmentation of...
Use the Thermo Scientific™ Pierce™ HeLa Protein Digest Standard, a highly validated mammalian protein digest, as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.
Calibrate and maintain quality control for liquid chromatography (LC) and mass spectrometry (MS) analysis of proteomic samples using the carefully validated Thermo Scientific™ Pierce™ BSA Protein Digest Standard, LC-MS Grade.
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Learn about methods and technologies for peptide and protein analysis by high performance liquid chromatography and mass spectrometry (LC/MS). Understand sample prep tools, mass and isotope labeling strategies for quantitative proteomics, and the basics of MS data and analysis.
View all What is the difference between Relative and Absolute Quantitation? Which type of quantitation is used for proteomic samples? What is the main purpose of Tandem Mass Tag (TMT) reagents? What type of mass spectrometer can I use for Tandem Mass Tag (TMT) analysis?
Documents & Support (131)
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Can I use protease inhibitors for mass spectrometry (MS) sample prep?
How can I determine peptide yield in my mass spectrometry samples after sample clean-up?