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In vitro Protein Expression Systems
These products include in vitro expression systems, plasmid controls, cell extracts, and vectors for cell-free translation, high-fidelity, recombinant protein expression, and post-translational modification from RNA or DNA templates.
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The Thermo Scientific™ 1-Step Human In Vitro Protein Expression Kits enable the translation and post-transcriptional modification of full-length proteins from mRNA or plasmid templates with yields of up to 100μg/mL per reaction.
The ExpiCHO Expression System Kit combines the power of rapid, ultra–high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster ovary (CHO) cells. It is based on high-density culture of ExpiCHO-S cells in ExpiCHO Expression Medium.
The Thermo Scientific™ 1-Step Human High-Yield In Vitro Translation (IVT) Kits with continuous-feed device enables the expression of functional proteins with 10 to 100 times greater yield per mL than other eukaryotic IVT translation systems.
Thermo Scientific™ T7 Cell-Free Expression Vectors (pT7CFE1) are cloning plasmids optimized to use with the Thermo Scientific 1-Step Human In Vitro Protein Expression System for in vitro translation (IVT) of tagged fusion proteins.
The ExpiSf Expression System is a complete chemically-defined baculovirus-insect cell protein expression system that delivers superior yields and consistent performance run after run using a fast, streamlined workflow.
The ExpiSf Protein Production Kit is designed to significantly boost protein expression from high-density suspension cultures of Sf9 insect cells. The kit includes the chemically defined, animal origin-free ExpiSf Enhancer and ExpiSf CD Medium, which together power the highest possible level of...
Expi293 Inducible Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells
Gibco™ ExpiSf™ CD Medium is chemically defined, yeastolate-free, serum-free, protein-free, animal origin-free medium specifically designed for high-density growth, transfection, and baculovirus infection of suspension-adapted Sf9 (Spodoptera frugiperda) insect cells.
Expi293™ MembranePro™ Expression System
Thermo Scientific™ T7 Cell-Free Expression Vectors (pT7CFE1) are cloning plasmids optimized to use with the Thermo Scientific 1-Step Human In Vitro Protein Expression System for in vitro translation (IVT) of tagged fusion proteins.
BacMam LRRK2 Reagent
MembranePro™ Functional Protein Expression Kit - 10rxn
Protein Expression Sample Prep Kit
Bac-to-Bac™ N-His TOPO™ Expression System
For customers studying GPCRs and other membrane proteins, MembranePro™ is the ready-to-use system that delivers enriched, functional membrane proteins efficiently and reliably.
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Atul Deshpande, Ph.D.; Krishna Vattem, Ph.D., The Thermo Scientific 1-Step Human Coupled IVT Kit (Part No. 88881 ) is a mammalian in vitro translation (IVT) system based on HeLa cell lysates for use with DNA templates.
Eric Hommema, M.S.; Penny Jensen, Ph.D.; Krishna Vattem, Ph.D.; Brian Webb, Ph.D., In vitro protein expression is a rapid technique to produce functional proteins. By contrast with in vivo protein expression methods, in vitro protein expression allows for expression of toxic proteins, incorporation...
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What lysate systems can be used for in vitro protein expression?
What are the advantages of using a cell-free expression system?