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Streptavidin-Biotin Binding Products
Streptavidin is a protein that is used extensively in molecular biology applications due to binding tightly to biotin. These products include streptavidin conjugates for labeling biotin-binding proteins.
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Alexa Fluor™ 647 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye).
Alexa Fluor™ 488 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye).
Qdot™ 585 Streptavidin Conjugate
Streptavidin from Streptomyces avidinii conjugated to highly purified horseradish peroxidase (HRP)
A bacterially derived biotin-binding protein that reportedly exhibits less nonspecific binding than avidin.
Commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.
Thermo Scientific™ Pierce High Sensitivity Streptavidin HRP Conjugate is an exclusive peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.
Qdot™ 525 Streptavidin Conjugate
Streptavidin, Alexa Fluor™ 700 conjugate
Commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.
Streptavidin, Alexa Fluor™ 546 conjugate
Invitrogen™'s Alexa Fluor 430 streptavidin conjugate is a bright, green-fluorescent probe (absorption/emission maxima ∼434/539 nm).
Streptavidin, Alexa Fluor™ 635 conjugate
Commonly used in indirect staining protocols to detect biotinylated primary antibodies in flow cytometry. Streptavidin binds to biotin with high affinity.
LanthaScreen™ Eu-Streptavidin
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Streptavidin-based amplification techniques are widely used in flow cytometry, fluorescent imaging, western blotting, and microplate-based detection for increased signal output and greater sensitivity.
Invitrogen eFluor 660 dye is a red-fluorescent dye configured as an alternative to Alexa Fluor 647 or APC. The spectral properties make it an ideal fluorophore that can be used in any flow cytometry panel incorporating the red laser.
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My protein-coated microspheres appear to be non-specifically binding in my experimental system. Do you have a product that can help reduce these non-specific interactions?
In the ReadyProbes Streptavidin/Biotin Blocking Solution (1X) protocol, why is there a step to add biotin to the sample?