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Flow Cytometry Compensation, Counting and Calibration
Reagents and kits for flow cytometer instrument calibration, sample compensation beads and kits, cell counting standards, and other reagents needed to set your instrument up.
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Designed for use in compensation with all fluorochromes excited by ultraviolet, violet, blue, green, yellow-green, and red lasers. UltraComp eBeads™ react with antibodies of mouse, rat, and hamster origin, and are immunoglobulin light chain-independent. 1 drop (50μL)/test.
CountBright™ Absolute Counting Beads, for flow cytometry
Calibrated suspension of ∼4 μm beads that encapsulate dyes that are excitable across a wide range of UV-IR wavelengths and brightly fluoresce in the emission range of 385-860 nm.
Formulated and optimized for staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines
ArC™ Amine Reactive Compensation Bead Kit (for use with LIVE/DEAD™ Fixable dead cell stain kits)
For staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines. Should be used with the Permeabilization Buffer. All three components sold together as the Foxp3 / Transcription Factor Staining Buffer Set.
Can be used to fix cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333) when performing intracellular staining of antigens located in the cytoplasm and secretory pathway, such as cytokines, chemokines and growth factors.
Provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated mouse, rat, hamster, or rabbit antibodies with any laser in flow cytometry.
BrightComp eBeads compensation beads provide a consistent, accurate, and simple-to-use technique for setting flow cytometry compensation when using fluorescent protein-expressing samples.
Spherical particles that can be stained with individual fluorochrome-conjugated antibodies for use as single-color compensation controls. OneComp eBeads react with antibodies of mouse, rat and hamster origin, and are immunoglobulin light chain-independent. 1 drop (50μL)/test
Flow Cytometry Size Calibration Kit (nonfluorescent microspheres)
Intended to be used with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.
Alignflow™ Flow Cytometry Alignment Beads for Blue Lasers, 6.0 μm
The eBioscience™ Mouse Intracellular (cytoplasmic/cytokine) Protein Flow Cytometry Workflow Kit contains all of the normal buffers, blockers, and beads needed to complete a standard flow cytometry experiment for staining intracellular cytoplasmic and cytokine targets in mouse cell suspensions.
Recommended for use as positive control cells when establishing an intracellular cytokine staining protocol.
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Compensation beads capture species-specific antibodies conjugated to fluorophores and other types of reagents. The purpose of these beads is to set voltages and gating parameters for obtaining accurate fluorescence signal.
(See a list of the products featured in this article.) The discovery in the early 1960s [1] and subsequent development of Green Fluorescent Protein (GFP) as a reporter gene has greatly advanced the study of gene expression, protein localization, and cell and tissue development in a multitude of...
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What is compensation?
What is flow cytometry?