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One-Step Reverse Transcription PCR Reagents
Products that facilitate fast and convenient workflow by allowing the reverse transcription and subsequent amplification steps to be performed in a single reaction.
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The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance.
The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR.
The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.
Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.
Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube. It combines the high-processivity SuperScript IV Reverse Transcriptase (RT) with a novel UniPrime RT-PCR Master Mix to provide...
Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.
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Reverse transcription is used to investigate the functions of RNA. RNA is routinely converted to more stable complementary DNA (cDNA) by reverse transcription (RT). cDNA allows further manipulations to study RNA using DNA-based techniques such as cloning, PCR, and sequencing, so reverse...
Reverse transcriptases (RTs) are RNA-dependent DNA polymerases, a group of enzymes that play a unique role in the flow of genetic information. These enzymes enable the reverse transcription reaction and have been widely used by researchers in a variety of molecular biology applications since their...
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Does Power SYBR Green PCR Master Mix (Cat. No. 4368577, 4367659, 4368706, 4368702, 4368708, 4367660) work with reverse transcription (RT)-PCR?
What percentage of RNA is converted to cDNA when performing reverse transcription?