LAMP and Other Isothermal Amplification Technologies
Isothermal amplification covers multiple technologies, including loop-mediated isothermal amplification (LAMP), that are used to amplify nucleic acids (RNA and DNA) exponentially at constant temperature, eliminating the need for thermocycler equipment. Isothermal amplification techniques are suitable for detecting various pathogens from environment, plants, human and animal samples.
Invitrogen SuperScript IV RT-LAMP Master Mix is a ready-to-use solution optimized for fast, sensitive, and simple RNA target detection in loop-mediated isothermal amplification (LAMP) reactions.
Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors. By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal...
The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).
This reaction buffer is for use with EquiPhi29 DNA Polymerase. Buffer is included with purchase of the polymerase, but additional buffer is made available here to help accommodate experimental needs.
Thermo Scientific Bsm DNA Polymerase, Large Fragment, is equivalent to Bst DNA polymerase, which catalyzes 5' → 3' synthesis of DNA and lacks 5' → 3' and 3' → 5' exonuclease activities. Sourced from E.
Invitrogen Lyo-ready recombinase polymerase amplification (RPA) reagents are designed for fast, sensitive, and specific DNA amplification through isothermal RPA reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.
Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification. Produced from E. coli cells with a cloned gene 2 of Bacillus subtilis phage phi29, phi29 DNA Polymerase possesses...
Loop-mediated isothermal amplification, also known as LAMP, is a simple and cost-effective nucleic acid amplification technique that does not require expensive instruments and helps ensure rapid results and easy outcome interpretation.
Isothermal nucleic acid amplification is a technique used to amplify specific DNA or RNA sequences at a constant temperature, typically between 37–65°C, eliminating the need for the thermal cycling equipment utilized in traditional PCR ( p olymerase c hain r eaction).