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LAL Endotoxin Testing
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Endotoxin is a lipopolysaccharide (LPS) that constitutes much of the outer membrane of the cell wall in gram-negative bacteria. LPS is the most abundant antigen on the cell surface of most gram-negative bacteria.
The Invitrogen Quant-iT Endotoxin Detection Assay Kit is a sensitive and easy-to-use fluorescent endpoint assay for use on fluorescence microplate readers that uses amebocyte lysates to quantify endotoxin in protein, peptide, antibody, or nucleic acid samples.
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Endotoxin contamination is a common problem with recombinant proteins and nucleic acids purified from gram-negative bacteria such as E. coli. Endotoxins are lipopolysaccharides (LPS), which are heat-stable molecules associated with the outer membranes of certain gram-negative bacteria.
Endotoxins are heat-stable lipopolysaccharide (LPS) molecules associated with the outer membranes of certain gram-negative bacteria, such as E. coli. Endotoxins are routinely released by bacteria as they grow and divide in their normal life cycle, but large volumes of endotoxins can be released...
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I am using the Thermo Scientific LAL Chromogenic Endotoxin Quantitation Kit. How do I convert from EU (Endotoxin Unit) to nanogram?
With the Thermo Scientific LAL Chromogenic Endotoxin Quantitation Kit, what is assay inhibition and how should I check for it?