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Tris-Glycine Transfer Buffer (25X) Invitrogen™

Tris-Glycine Transfer Buffer (25X) is optimized for western blot transfer of proteins to nitrocellulose, PVDF, or nylon membranes for subsequent analysis using Tris-Glycine gels. To use: This buffer should be diluted to a 1X solution with a water/methanol mixture to yield a final methanol...

Pierce™ Western Blot Signal Enhancer Thermo Scientific™

Thermo Scientific™ Pierce Western Blot Signal Enhancer is a two-reagent system for conditioning protein blots after transfer to greatly enhance the effectiveness of primary antibodies and intensify the final detection signal in Western blot experiments.

Restore™ PLUS Western Blot Stripping Buffer Thermo Scientific™

Thermo Scientific™ Restore PLUS Western Blot Stripping Buffer is an advanced formula for removing bound primary and secondary antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels) Invitrogen™

The NuPAGE MES SDS Buffer Kit is designed for separation of small- to medium-size proteins on NuPAGE Bis-Tris gels and includes the following buffers: • NuPAGE MES SDS Running Buffer (20X, 500 mL, Cat. No. NP0002) • NuPAGE Sample Reducing Agent (10X, 250 μL, Cat. No.

SuperSignal™ Western Blot Enhancer Thermo Scientific™

Thermo Scientific™ SuperSignal Western Blot Enhancer contains a membrane treatment reagent and a primary antibody diluent that increase both signal intensity and sensitivity 3- to 10-fold compared to detection performed without it.

WesternBreeze™ Blocker/Diluent (Part A and B) Invitrogen™

The WesternBreeze® Blocker/Diluent (part A and B) is an optimized, easy-to-use blocker and primary antibody diluent system that yields low background/high signal western blot detection on nitrocellulose (NC) and polyvinylidene difluoride (PVDF) membranes. Sufficient reagents for 20 mini-blots.

Pierce™ 1-Step Transfer Buffer Thermo Scientific™

The Thermo Scientific™ Pierce 1-Step Transfer Buffer is designed for rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.

NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels) Invitrogen™

The NuPAGE MOPS SDS Buffer Kit is designed for separation of medium- to large-size proteins on NuPAGE Bis-Tris gels and includes the following buffers: • NuPAGE MOPS SDS Running Buffer (20X, 500 mL, Cat. No. NP0001) • NuPAGE Sample Reducing Agent (10X, 250 μL, Cat. No.

WesternBreeze™ Chromogenic Kit, anti-rabbit Invitrogen™

WesternBreeze® Chromogenic Kits detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots). Detection is accomplished with a ready-to-use BCIP/NBT substrate for alkaline phosphatase.

Restore™ Fluorescent Western Blot Stripping Buffer Thermo Scientific™

Thermo Scientific™ Restore Fluorescent Western Blot Stripping Buffer is a gentle and highly effective reagent for quickly removing primary and near-infrared (IR) dye-labeled secondary antibodies from Western blots.

Novex™ Tris-Glycine SDS Buffer Kit Invitrogen™

Pre-mixed buffers are a convenient way to ensure high-quality, consistent electrophoresis results. All buffers are made with high-purity reagents and are strictly quality controlled. The buffers are provided as a concentrated solution requiring a simple dilution with deionized water before use.

iBind™ Western Starter Kit Invitrogen™

The iBind Western Starter Kit includes an iBind Western Device as well as all of the consumables and reagents necessary to get started using this automated western-processing device. The iBind Western Device performs every step in the western protocol from blocking to washes to antibody incubations...

WesternBreeze™ Wash Solution (16X) Invitrogen™

WesternBreeze® Wash Solution (16X) is an optimized solution of concentrated buffered saline containing detergent to minimize background and non-specific binding on nitrocellulose (NC) and polyvinylidene difluoride (PVDF) membranes. Sufficient reagents for 20 mini-blots.

Incubation Tray, 10 x 14 cm Invitrogen™

The Incubation Tray enables convenient and even staining of E-PAGE™ blots. The tray is 10 cm (l) x 14 cm (w) x 3 cm (d) with a lid. It is suitable for use with immunodetection or blot stains.

ZOOM™ CHAPS Invitrogen™

The ZOOM™ IEF Fractionator offers a fast, reliable method to reduce sample complexity, enrich low abundance proteins, and increase the dynamic range of detection. Solution phase isoelectric focusing with the ZOOM™ IEF Fractionator provides reproducible separations in three hours.

General Western Blot Reagents