Taq DNA Polymerase PCR Buffer is a 10X buffer [200 mM Tris HCl (pH 8.4), 500 mM KCl] supplied with 1 ml of 50 mM MgCl2. It is included with Platinum™ Taq, Taq, and the SuperScript™ First-Strand Synthesis System for RT-PCR.
PCRx Enhancer System is used in conjunction with DNA polymerases to optimize PCR of problematic and/or GC-rich templates (1). 10X PCRx Enhancer Solution with 10X PCRx Amplification Buffer offers higher primer specificity, broader magnesium concentration, broader annealing temperature, and improved...
Applied Biosystems™ GeneAmp™ 10X PCR Buffer I is optimized for use with AmpliTaq™ DNA Polymerase, resulting in robust PCR amplification. • Optimized for use with AmpliTaq™ DNA Polymerase, offering maximum PCR performance • Available in several convenient sizes GeneAmp™ 10X PCR Buffer I contains 15...
M-MLV Reverse Transcriptase Buffer is for use with Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT). 5X buffer composition • 250 mM Tris-HCl (pH 8.3) • 375 mM KCl • 15 mM MgCl2 Included with • M-MLV Reverse Transcriptase • SuperScript Reverse Transcriptase • SuperScript II Reverse...
SuperScript IV RT Reaction Buffer is included with, and designed for use with, SuperScript IV Reverse Transcriptase. This buffer is optimized to give robust and reliable cDNA synthesis. In cases where additional quantity is required, SuperScript IV RT Reaction Buffer is made available here in a...
For the polyadenylation of in vitro transcribed RNA to enhance translation initiation efficiency. Sufficient reagents are included for 25 reactions. • Adds a poly(A) tail of at least 150 nucleotides in length to the 3' termini of RNA • Enhances translational efficiency in vivo • Reaction can be...
Guanidine thiocyanate is a useful tool for RNA isolation and protein solubilization. It is involved in the quantification of mRNA from hepatocytes. It acts as a medium for blood smaple lystae in order to maintain the integrity of nucleic acid.
Invitrogen™ SuperFi™ Buffer is a 5X buffer solution optimized for use with Invitrogen™ Platinum™ SuperFi DNA Polymerase. The buffer contains 7.5 mM MgCl2 which provides 1.5 mM MgCl2 in the final reaction.
Invitrogen™ SuperFi™ Green Buffer is a 5X buffer solution optimized for use with Invitrogen™ Platinum™ SuperFi DNA Polymerase. The buffer contains 7.5 mM MgCl2 which provides 1.5 mM MgCl2 in the final reaction.
PCR (Polymerase Chain Reaction) is used in a wide variety of applications including cloning, gene expression analysis, genotyping, sequencing, and mutagenesis. The Thermo Scientific PCR portfolio has PCR and qPCR consumables for every successful PCR run: thermal cyclers, plates, tubes, sealing...
Invitrogen Platinum II Taq Hot-Start DNA Polymerase is designed to get you to your research destination, faster. A universal primer annealing feature reduces optimization steps and allows for co-cycling of different assays.
With Platinum II Taq Hot-Start DNA Polymerase and Platinum II PCR buffer, how is it possible to use an annealing temperature of 60 degrees C for any primer pair?