Crosslinking Reagents

The heterobifunctional, crossslinker succinimidyl 3-(2-pyridyldithio)propionate (SPDP) enables one to covalently link two target groups. The succinimidyl ester chemically reacts with primary amines and after mild reduction of the dithiol, it can react form a disulfide with other thiols.

EZ-Link Biotin-PEG12-DBCO is a biotinylation reagent that contains a dibenzylcyclooctyne (DBCO) group for copper-free click chemistry attachment to an azide-labeled molecule, enabling the attachment of biotin using a bioorthogonal approach.

EZ-Link TFP Ester-PEG12-DBCO is a hetero-bifunctional crosslinker that contains an amine-reactive tetrafluorophenyl (TFP) group and a dibenzylcyclooctyne (DBCO) group with a water-solubilizing long PEG12 spacer in between.

Thermo Scientific Pierce SM(PEG)12 is a hetero-bifunctional crosslinker with N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules.

Thermo Scientific Pierce SM(PEG)2 is a hetero-bifunctional crosslinker with N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules.

Thermo Scientific™ Pierce NHS-Azide reagents are amine-reactive compounds that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to phosphine-modified molecules.

Thermo Scientific™ Pierce NHS-Phosphine and Sulfo-NHS-Phosphine are amine-reactive compounds that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to azide-tagged molecules.

Thermo Scientific™ Pierce TSAT is tris(succinimidyl)aminotriacetate, a novel trifunctional crosslinker that contains three short spacers arms linked to a central nitrogen atom, each ending in an amine-reactive NHS-ester group.

Thermo Scientific™ Pierce TMEA is a trifunctional crosslinker containing three reactive maleimide groups to enable three-way conjugation and polymerization of sulfhydryl-containing molecules.

Thermo Scientific™ Pierce PMPI is a maleimide-and-isocyanate crosslinker for attaching compounds to sulfhydryl groups (cysteines) after conjugating the linker to hydroxyl groups on a molecule by urethane (carbamate) bonds.

Thermo Scientific™ Pierce NHS-Diazirine Crosslinkers combine proven NHS-ester and diazirine-based photoreaction chemistries to conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation.

Thermo Scientific™ Pierce NHS-Diazirine Crosslinkers combine proven NHS-ester and diazirine-based photoreaction chemistries to conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation.

Thermo Scientific™ Pierce NHS-Diazirine Crosslinkers combine proven NHS-ester and diazirine-based photoreaction chemistries to conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation.

Thermo Scientific™ Pierce NHS-Diazirine Crosslinkers combine proven NHS-ester and diazirine-based photoreaction chemistries to conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation.

Thermo Scientific™ Pierce NHS-Diazirine Crosslinkers combine proven NHS-ester and diazirine-based photoreaction chemistries to conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation.

Thermo Scientific™ Pierce NHS-Diazirine Crosslinkers combine proven NHS-ester and diazirine-based photoreaction chemistries to conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation.

Thermo Scientific™ Pierce NHS-Azide reagents are amine-reactive compounds that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to phosphine-modified molecules.

Thermo Scientific™ Pierce PEG12-SPDP is a multifunctional crosslinker for protein conjugation via amine-to-amine or amine-to-sulfhydryl crosslinks that contain a 12-unit polyethylene glycol (PEG) group and a reducible (cleavable) disulfide bond.

Thermo Scientific™ Pierce PEG4-SPDP is a multifunctional crosslinker for protein conjugation via amine-to-amine or amine-to-sulfhydryl crosslinks that contain a 4-unit polyethylene glycol (PEG) group and a reducible (cleavable) disulfide bond.

Thermo Scientific™ Pierce NHS is pure N-hydroxysuccinimide, the essential compound needed to prepare amine-reactive NHS-ester crosslinkers or to enhance conjugation efficiencies when using the carbodiimide EDC.

Thermo Scientific™ Pierce Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes.

Thermo Scientific™ Pierce SPB is an NHS-ester and psoralen crosslinker that effectively conjugates primary amines to DNA via light-activated intercalation next to pyrimidine bases.

Thermo Scientific™ Pierce Photoreactive Amino Acids are analogs of L-Leucine and L-Methionine that can be incorporated into proteins during synthesis and then light-activated to covalently crosslink interacting proteins in cells.

Thermo Scientific™ Pierce Photoreactive Amino Acids are analogs of L-Leucine and L-Methionine that can be incorporated into proteins during synthesis and then light-activated to covalently crosslink interacting proteins in cells.

Thermo Scientific™ Pierce SMPB is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length aromatic spacer arm (11.6 Ås).

Thermo Scientific™ Pierce SMPH is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long spacer arm (14.2 Ås).

Thermo Scientific™ Pierce LC-SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a long cyclohexane-stabilized spacer arm (16.2 Ås)

Thermo Scientific™ Pierce SIA is a very short (1.5 Å) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and iodoacetyl reactive groups.

Thermo Scientific™ Pierce SBAP is a short (6.2 Å) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and bromoacetyl reactive groups.

Thermo Scientific™ Pierce BM(PEG)3 is a pegylated crosslinker having the form maleimide-PEG-maleimide for conjugation between sulfhydryl groups (e.g., cysteines) via a three-unit polyethylene glycol (PEG) spacer arm (17.8 Ås).