PCR Specific Buffers

Buffers optimized for specific PCR procedures. Products are available in various volumes and concentrations and for different PCR-related processes.
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Taq DNA Polymerase PCR Buffer is a 10X buffer [200 mM Tris HCl (pH 8.4), 500 mM KCl] supplied with 1 ml of 50 mM MgCl2. It is included with Platinum™ Taq, Taq, and the SuperScript™ First-Strand Synthesis System for RT-PCR.
Thermo Scientific™ 10X Taq Buffer without Detergent is used in PCR with Fermentas Taq DNA Polymerases (both recombinant and native), and this buffer does not contain detergents which can interfere with certain applications, e.g. microarray experiments. Additional Information and Online Ordering
DreamTaq™ Buffer (10X) Thermo Scientific™
Thermo Scientific™ 10X N321DreamTaq Green Buffer is a proprietary PCR buffer which contains KCl and (NH&subscript_4;)&subscript_2;SO&subscript_4; at a ratio optimized for robust performance of DreamTaq DNA Polymerase in PCR and includes MgCl&subscript_2; at a concentration of 20mM
PCRx Enhancer System is used in conjunction with DNA polymerases to optimize PCR of problematic and/or GC-rich templates (1). 10X PCRx Enhancer Solution with 10X PCRx Amplification Buffer offers higher primer specificity, broader magnesium concentration, broader annealing temperature, and improved...
The Ambion™ Buffer Kit provides high quality, RNase free buffers for critical RNA analysis procedures. The kit consists of one 100 mL bottle of each of the following solutions: 1 M Tris pH 7.0, 1 M Tris pH 8.0, 5 M NaCl, 1 M MgCl2, 0.5 M EDTA pH 8.
GeneAmp™ 10X PCR Buffer Applied Biosystems™
Applied Biosystems™ GeneAmp™ 10X PCR Buffer I is optimized for use with AmpliTaq™ DNA Polymerase, resulting in robust PCR amplification. • Optimized for use with AmpliTaq™ DNA Polymerase, offering maximum PCR performance • Available in several convenient sizes GeneAmp™ 10X PCR Buffer I contains 15...
Thermo Scientific™ 10X Taq Buffer without Detergent is used in PCR with Fermentas Taq DNA Polymerases (both recombinant and native), and this buffer does not contain detergents which can interfere with certain applications, e.g. microarray experiments. Additional Information and Online Ordering
M-MLV Reverse Transcriptase Buffer is for use with Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT). 5X buffer composition • 250 mM Tris-HCl (pH 8.3) • 375 mM KCl • 15 mM MgCl2 Included with • M-MLV Reverse Transcriptase • SuperScript Reverse Transcriptase • SuperScript II Reverse...
Thermo Scientific™ 10X Taq Buffer without Detergent is used in PCR with Fermentas Taq DNA Polymerases (both recombinant and native), and this buffer does not contain detergents which can interfere with certain applications, e.g. microarray experiments. Additional Information and Online Ordering
PCR reagents meet high quality standards to generate accurate results from even the most challenging molecular biology applications. Magnesium ions (Mg2+) are an important component of PCR reactions where they interact with the DNA template, dNTPs, and Taq DNA Polymerase.
Phusion Green GC Buffer Pack Thermo Scientific™
Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases: • 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free)) • 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free)) The error rate of Phusion DNA Polymerase...
Phusion GC Buffer Pack Thermo Scientific™
The Thermo Scientific™ Phusion™ GC Buffer Pack is a separate GC buffer pack optimized for use with Phusion DNA polymerases.
Thermo Scientific™ 10X N321DreamTaq Green Buffer is a proprietary PCR buffer which contains KCl and (NH&subscript_4;)&subscript_2;SO&subscript_4; at a ratio optimized for robust performance of DreamTaq DNA Polymerase in PCR and includes MgCl&subscript_2; at a concentration of 20mM
Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases: • 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free)) • 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free)) The error rate of Phusion DNA Polymerase...
SuperScript IV RT Reaction Buffer is included with, and designed for use with, SuperScript IV Reverse Transcriptase. This buffer is optimized to give robust and reliable cDNA synthesis. In cases where additional quantity is required, SuperScript IV RT Reaction Buffer is made available here in a...
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PCR (Polymerase Chain Reaction) is used in a wide variety of applications including cloning, gene expression analysis, genotyping, sequencing, and mutagenesis. The Thermo Scientific PCR portfolio has PCR and qPCR consumables for every successful PCR run: thermal cyclers, plates, tubes, sealing...
Invitrogen Platinum II Taq Hot-Start DNA Polymerase is designed to get you to your research destination, faster. A universal primer annealing feature reduces optimization steps and allows for co-cycling of different assays.
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With Platinum II Taq Hot-Start DNA Polymerase and Platinum II PCR buffer, how is it possible to use an annealing temperature of 60 degrees C for any primer pair?
I would like to calibrate my Applied Biosystems ViiA 7 Real-Time PCR instrument with a custom dye, which buffer should I use?