These products include in vitro expression systems, plasmid controls, cell extracts, and vectors for cell-free translation, high-fidelity, recombinant protein expression, and post-translational modification from RNA or DNA templates.
1-step Human Coupled IVT Kit (2)
1-step Human High-yield Maxi IVT Kit (1)
1-step Human High-yield Mini IVT Kit (2)
BacMam LRRK2 Reagent (1)
Cell Lysis Buffer (1)
Expression System (5)
Inducible Expression System Kit (1)
Insect Cell Protein Expression System (2)
Media and reagents, ExpiSf CD Meidum and ExpiSf Enhancer (3)
The Thermo Scientific™ 1-Step Human High-Yield In Vitro Translation (IVT) Kits with continuous-feed device enables the expression of functional proteins with 10 to 100 times greater yield per mL than other eukaryotic IVT translation systems.
The ExpiCHO™ Expression System Kit combines the power of rapid, ultra–high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster ovary (CHO) cells. It is based on high-density culture of ExpiCHO-S™ cells in ExpiCHO™ Expression Medium.
The Thermo Scientific™ 1-Step Human In Vitro Protein Expression Kits enable the translation and post-transcriptional modification of full-length proteins from mRNA or plasmid templates with yields of up to 100μg/mL per reaction.
The ExpiSf Protein Production Kit is designed to significantly boost protein expression from high-density suspension cultures of Sf9 insect cells. The kit includes the chemically defined, animal origin-free ExpiSf Enhancer and ExpiSf CD Medium, which together power the highest possible level of...
The Expi293 Inducible Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells. The system kit is centered on engineered Expi293F cells that stably express the tetracycline repressor (TetR) protein, thus enabling...
The Thermo Scientific™ 1-Step Human In Vitro Protein Expression Kits enable the translation and post-transcriptional modification of full-length proteins from mRNA or plasmid templates with yields of up to 100μg/mL per reaction.
The Expi293 GnTI- Expression System Kit is part of the Expi293 platform for rapid, high-yield transient protein expression from mammalian 293 cells. The system kit is centered on engineered Expi293F cells that do not have N-acetylglucosaminyltransferase I (GnTI) activity and therefore lack complex...
Bac-to-Bac baculovirus expression is an efficient method for producing baculovirus for high-level protein expression in insect cells. It relies on generation of recombinant virus by site-specific transposition in E. coli rather than homologous recombination in insect cells.
The Applied Biosystems® Protein Expression Sample Preparation Kit contains the reagents required for the preparation of target and control cell lysates for use with TaqMan® Protein Expression Assay experiments.
The PichiaPink™ Secreted Protein Expression Kit is a new recombinant protein expression system based on the yeast Pichia pastoris. The PichiaPink™ Secreted Protein Expression Kit gives you convenient and cost-efficient bioproduction from shake flask to 1000-liter production of your recombinant...
Bac-to-Bac baculovirus expression is an efficient method for producing baculovirus for high-level protein expression in insect cells. It relies on generation of recombinant virus by site-specific transposition in E. coli rather than homologous recombination in insect cells.
Thermo Scientific™ T7 Cell-Free Expression Vectors (pT7CFE1) are cloning plasmids optimized to use with the Thermo Scientific 1-Step Human In Vitro Protein Expression System for &italic_begin;in vitro&italic_end; translation (IVT) of tagged fusion proteins.
For customers studying GPCRs and other membrane proteins, MembranePro™ is the ready-to-use system that delivers enriched, functional membrane proteins efficiently and reliably. Benefits of MembranePro™ include: • Proteins are displayed on human (or other mammalian) cell membranes • Cellular quality...
The ExpiSf Expression System is a complete chemically-defined baculovirus-insect cell protein expression system that delivers superior yields and consistent performance run after run using a fast, streamlined workflow.
ExpiSf CD Medium is a GMP-manufactured, chemically defined, yeastolate-free, serum-free, protein-free, animal origin-free medium specifically designed for high-density growth, transfection, and baculovirus infection of suspension-adapted Sf9 (Spodoptera frugiperda) insect cells.
Atul Deshpande, Ph.D.; Krishna Vattem, Ph.D., The Thermo Scientific 1-Step Human Coupled IVT Kit (Part No. 88881 ) is a mammalian in vitro translation (IVT) system based on HeLa cell lysates for use with DNA templates.
Eric Hommema, M.S.; Penny Jensen, Ph.D.; Krishna Vattem, Ph.D.; Brian Webb, Ph.D., In vitro protein expression is a rapid technique to produce functional proteins. By contrast with in vivo protein expression methods, in vitro protein expression allows for expression of toxic proteins, incorporation...