cDNA Synthesis Kits and Master Mixes

A selection of kits and master mixes providing all required components for first strand cDNA synthesis.
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SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications. The inclusion of ezDNase enzyme further accelerates the RT-qPCR workflow through an extremely simplified...
Invitrogen SuperScript IV Template-Switching RT Master Mix is designed for high template switching efficiency in cDNA synthesis reactions. It contains SuperScript IV Reverse Transcriptase, RNase Inhibitor, and all other reverse transcription reaction components in a convenient premixed format.
The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.
The SuperScript III First-Strand Synthesis SuperMix is an optimized formulation for first-strand cDNA synthesis from purified poly(A)+ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system. The amount of starting material can vary from 0.1 pg to 5 μg of total RNA.
Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit uses RevertAid Reverse Transcriptase (RT), a recombinant M-MuLV RT which maintains activity at 42–50°C and is suitable for synthesis of cDNA up to...
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA. The system includes SuperScript IV Reverse Transcriptase (RT), a proprietary MMLV mutant that produces robust and...
TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.
The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes.
The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.
The SuperScript III First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system and the amount of starting material can vary from 1 pg to 5 μg of total RNA.
The Thermo Scientific Maxima First Strand cDNA Synthesis Kit for RT-qPCR, available with or without dsDNase, is a convenient system optimized for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications.
The SuperScript IV CellsDirect cDNA Synthesis Kit is optimized for the synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating RNA. Cell lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in PCR and...
Thermo Scientific Maxima H Minus First Strand cDNA Synthesis Kit, available with or without dsDNase, is a complete system for highly efficient synthesis of first strand cDNA. The kit uses the Maxima H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by in vitro evolution of MMLV...
SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications. The master mix combines SuperScript III Reverse Transcriptase (RT) and other RT reaction components in a single-tube format to enable reliable first-strand synthesis and high cDNA yields from a...
The SuperScript First-Strand Synthesis System for RT-PCR is optimized for the synthesis of first-strand cDNA from purified poly(A)+ or total RNA. The system can be used with as little as 1 ng or as much as 5 μg of total RNA.
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The direct PCR approach delivers convenience for DNA amplification by allowing PCR directly from samples without prior DNA purification. Significant savings in time and cost can be achieved in applications such as plant and animal genotyping.
The Thermo Scientific portfolio offers everything needed for successful PCR: A range of enzymes in stand-alone and master mix formats for routine, hot-start, and high-fidelity applications; direct PCR kits and master mixes for amplification without DNA purification; and buffers and reagents to...
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How does SuperScript IV VILO Master Mix perform with GC-rich RNA templates?
How does SuperScript IV VILO Master Mix perform with GC-rich RNA templates?