DoubleDigest Calculator

DoubleDigest Calculator is an easy tool for molecular biologists aiming to perform double digestion setups with restriction enzymes. This online calculator simplifies the process of determining optimal reaction conditions when using two restriction enzymes simultaneously. By inputting two specific restriction enzymes, the tool helps provide double digestion setup recommendations, buffer selection, incubation times, and reaction conditions, helping ensure efficient and accurate DNA digestion. This aids researchers in streamlining their workflows, helping reduce trial and error and achieve reliable results in their molecular cloning and genetic analysis experiments.

Easily determine optimal reaction conditions for your double digest reaction using this tool. DoubleDigest calculator conveniently calculates the right enzymatic reaction buffer, enzyme concentrations, incubation conditions, and any additives needed in your double digest reaction. Peak DNA digestion without star activity is best accomplished with conventional Thermo Scientific restriction enzymes using the Five Buffer System. For fast DNA digestion in a single buffer, upgrade to Thermo Scientific FastDigest restriction enzymes.