Technical Resources

McCoy's 5A Medium (modified) (1X), liquid

Contains L-glutamine.

McCoy's Media were originally formulated for the propagation of human lymphocytes. McCoy's 5A medium (modified) was based on the original formulation of McCoy as modified by Hsu and Kellog and further modified by Iwakata and Grace.

  • Catalog Number(s)

ComponentsMolecular WeightConcentration (mg/L)mM
Amino Acids
Glycine75.07.50.1
L-Alanine89.013.90.15617977
L-Arginine hydrochloride211.042.10.19952606
L-Asparagine132.045.00.3409091
L-Aspartic acid133.019.970.15015037
L-Cysteine121.031.50.2603306
L-Glutamic Acid147.022.10.15034014
L-Glutamine146.0219.21.5013698
L-Histidine hydrochloride-H2O210.020.960.09980952
L-Hydroxyproline131.019.70.15038168
L-Isoleucine131.039.360.300458
L-Leucine131.039.360.300458
L-Lysine hydrochloride183.036.50.19945355
L-Methionine149.014.90.099999994
L-Phenylalanine165.016.50.1
L-Proline115.017.30.15043478
L-Serine105.026.30.25047618
L-Threonine119.017.90.15042016
L-Tryptophan204.03.10.0151960775
L-Tyrosine181.018.10.1
L-Valine117.017.60.15042736
Vitamins
Ascorbic Acid176.00.50.0028409092
Biotin244.00.28.1967213E-4
Choline chloride140.05.00.035714287
D-Calcium pantothenate477.00.24.1928721E-4
Folic Acid441.010.00.022675738
Niacinamide122.00.50.0040983604
Nicotinic acid (Niacin)123.00.50.0040650405
Para-Aminobenzoic Acid137.01.00.00729927
Pyridoxal hydrochloride204.00.50.0024509805
Pyridoxine hydrochloride206.00.50.0024271845
Riboflavin376.00.25.319149E-4
Thiamine hydrochloride337.00.25.934718E-4
Vitamin B121355.02.00.0014760147
i-Inositol180.036.00.2
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.)111.0100.00.9009009
Magnesium Sulfate (MgSO4-7H2O)246.0200.00.8130081
Potassium Chloride (KCl)75.0400.05.3333335
Sodium Bicarbonate (NaHCO3)84.02200.026.190475
Sodium Chloride (NaCl)58.06460.0111.37931
Sodium Phosphate monobasic (NaH2PO4-H2O)138.0580.04.2028985
Other Components
Bacto-Peptone600.0Infinity
D-Glucose (Dextrose)180.03000.016.666666
Glutathione (reduced)307.00.50.0016286644
Phenol Red376.410.00.026567481

Reference:

1. Committee, In Vitro (1974) 9:6. 

2. Hsu, T.C. and Kellogg, D.S., Jr. (1960) J. Nat. Cancer Inst., 25:221.

3. Iwakata, S. and Grace, J.T., Jr. (1964) N.Y.J. Med., 64:2279.

4. McCoy, T.A., Maxwell, M. and Kruse, P.F. (1959) Proc. Soc. Exper. Biol. Med., 100:115. 

5. Sertich, G.J., Glass, J.R., Fuller, D.J.M., et al., (1986) J. Cell. Physiol., 127:114. (CHO adherent cells).

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