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Introduction to cryo-EM
Sample preparation
Introduction to the microscope
Image formation and aberrations
Accelerator
Condenser lens and parallel illumination
Objective, lens, stage, and projection
Detectors and cameras
Single particle analysis data collection and EPU
Entire workflow uninterrupted
Getting started in cryo-EM
Review of the different sources of the electrons in an electron microscope.
Overview of the stability of field emission electron guns and how they change over time.
Explanation and review of the gun shift theory.
Demonstration on the gun shift procedure.
Explanation and review of the gun tilt theory.
Demonstration and review of the FEG Registers and gun alignment in the TEM User Interface.
Theoretical review of the gun lens, their function and components.
Demonstration of changing the gun lens and performing a quick alignment in the TEM User Interface.
Step by step demonstration of the Gun alignment within the TEM User Interface.
For Research Use Only. Not for use in diagnostic procedures.