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Whether your primary sequencing technique is next-generation sequencing or capillary electrophoresis, you likely come across a question or two in your everyday sequencing workflow. Seq It Out is a new educational video series that sheds light on a variety of sequencing topics. We aim to provide bite-sized answers to the questions you have every day.
Click the button below to help guide the creation of new videos, by asking your own question about sequencing.
Today’s topic is the confirmation of next gen sequencing or NGS results using good old Sanger sequencing. You may ask “why on earth would I want to do that? This sounds really backwards!”
Why is a clean template for your Sanger sequencing reaction important? And what are the ways to get it without losing too much precious samples? Let’s find out today.
Sanger Sequencing is named after the inventor of this ground breaking technology, Dr. Frederick Sanger, who developed this method over 40 years ago in the mid-70s.
Ion Ampliseq Chemistry is like your favorite Swiss Army Knife. It is highly flexible and can be used across a multitude of applications. So what are the key applications for this targeted sequencing? Let’s find out today.
“What is Ion AmpliSeq™ Chemistry & how does it work?” Let’s find out what this new technology can do for you.
Whether you are new to next generation sequencing, or an old hand, you have likely encountered terms like base quality, Q-score, consensus accuracy and so on.
If you are new to Next Generation Sequencing, you’ve heard about the large amount of data it generates and the challenges with finding meaning in all that data.
Fragment analysis refers to a genetic analysis technique used for a wide variety of applications such as mutation detection, genotyping, DNA profiling, genetic mapping and linkage analysis.
So you are seeing some variants in your NGS data that you are not confident about. How can you quickly re-sequence a particular locus of interest?
NewCapillary Electrophoresis Instruments Support Center
Find tips, troubleshooting help, and resources for support related to your capillary electrophoresis instruments.
NEW | Next-Generation Sequencing Support Center Find tips, troubleshooting help, and resources for your next-gen sequencing applications. |
For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.