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Delivering allele-level resolution and speed in HLA genotyping
The AllType Rapid 11 Loci Kit combines Oxford Nanopore’s long-read, single-molecule sequencing, a simple workflow, and integrated, easy-to-use software to deliver fast, accurate, comprehensive high-resolution typing with very little hands-on time.
AllType Rapid produces data with high specificity, high read depth, and potentially less phase or cis-trans ambiguities in 11 HLA loci, with second-field concordance greater than 99% in as little as 4.5 hours for a single sample and 11 hours for multi-sample workflow (up to 24 samples).
Why choose the AllType Rapid NGS solution?
Flexible and rapid workflow
- Single and multiplex amplification
- Single-sample barcoded solution in < 4.5 hours
- Multi-sample workflow in < 11 hours
- Compatible with Oxford Nanopore Technologies:
– R10.4 Flow cell
– MinION Mk1B sequence device
Phased genotyping
- Fully phased Long-Range Sequence
- High-resolution
- Resolves 2nd field cis-trans ambiguities
Quality results
- High concordance
- Uniform data
- Balanced alleles
Workflow
The single-sample workflow is designed for speed and simplicity with minimal hands-on time while the multi-sample workflow is designed to allow for flexible and efficient throughput while still leveraging the speed and simplicity of nanopore sequencing.
Figure 1. AllType Rapid workflow.
* Sequencing time dependent on number of active pores.
** Analysis time dependent on number of CPU processing cores.
Analysis
Once sequencing on the Oxford Nanopore instrument is complete, our TypeStream Visual NGS Analysis software will automatically retrieve the data and begin analysis. As the base calling is live, you can see the files created in real time and stop the sequencer when there are enough reads, reducing the turnaround time for results.
By eliminating manual data transcription, the software simplifies data reporting and eliminates opportunities for errors.
For Research Use Only. Not for use in diagnostic procedures. Availability of product in each country depends on local regulatory marketing authorization status. Please consult your local sales representative for details. Oxford Nanopore Technologies, MinION, and MK1b, are trademarks of Oxford Nanopore Technologies plc.