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Neurobiology ELISA Kits and Multiplex Immunoassays |
Study proteins implicated in neurodegenerative disorders
Thermo Fisher Scientific offers a wide selection of sensitive and accurate immunoassays for neurobiology and neuroscience research. These assays include ELISA kits and multiplex immunoassays (for the Luminex technology platform) that allow quantification of biomarkers in cerebrospinal fluid (CSF), plasma, and serum for neuroinflammation, neurodegeneration, traumatic brain injury, and variety of conditions and diseases of the central nervous system (CNS).
Overview of neurobiology
Quantification of protein biomarkers is critical for neurobiology and neuroscience research and antibody-based techniques, such as enzyme-linked immunosorbent assays (ELISA) and multiplex immunoassays, offer powerful tools to achieve sensitive quantification. These proteins include neurodegenerative disease biomarkers, traumatic brain injury biomarkers, including those released due to astroglial, axonal, and neuronal cell body injury, neurotrophic factors (NTFs) that play a key role in the development of neurons, their plasticity, protection, repair, and key cytokines and chemokines that are involved in central nervous system (CNS) tissue homeostasis and neuroinflammation (Figure 1).
A variety of conditions or diseases of the CNS including brain trauma, stroke, multiple sclerosis, Parkinson’s disease, or Alzheimer’s disease can trigger neuroinflammation and dysfunction of blood-brain barrier (BBB). Acting in concert with aging, epigenetics, the environment, and other risk factors can strongly influence the onset and progression of these diseases. For example, traumatic brain injury has been established as a risk factor for the development of neurodegenerative diseases and dementia at later stages of life. In response to insults that perturb homeostasis in the CNS, a rapid and early activation of the glial cells form the acute inflammatory response. This leads to the repair of the damaged area but if the challenge is not cleared and persists, the activation of the response is exaggerated, becomes chronic, and can result in tissue degeneration and even the drastic loss of BBB integrity.
Key targets for neurodegeneration:
- Amyloid-beta (Aβ, beta amyloid, or Abeta), which is crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer’s patients.
- Tau, a protein which stabilizes microtubules and which, when not functioning properly, is implicated in pathologies and dementias of the nervous system.
- Alpha synuclein (α-synuclein), which is thought to play an important role in maintaining a supply of synaptic vesicles in presynaptic terminals and may also help regulate the release of dopamine.
Key targets for traumatic brain injury:
- Glial fibrillary acidic protein (GFAP) is a monomeric intermediate filament (IF) protein found in the astroglial cytoskeleton. This protein is not found outside the central nervous system and is only released after cell death or injury, when it may be detected in the blood.
- S100B is a calcium-sensor protein that is highly expressed in astrocytes but also present in other cells of the nervous system, including oligodendrocytes, Schwann cells, and ependymal cells. Elevated S100B levels in biological fluids has been shown to be a biomarker for acute brain injury.
- Neurofilament—Heavy (NF-H) and Light (NF-L) are subunits of the neurofilament scaffolding protein of neurons. Neuronal damage can cause the release of neurofilament into the interstitial fluid and subsequently to the cerebrospinal fluid and the blood.
- Ubiquitin C-terminal hydrolase (UCH-L1) is a brain-specific deubiquitinating enzyme. Elevated cerebrospinal fluid and blood concentrations of UCH-L1 have been associated with traumatic brain injury.
Key targets for neuroinflammation:
- Brain-derived neurotrophic factor (BDNF) is a neurotrophin that induces the maintenance, survival, plasticity, and neurotransmitter regulation of neurons, and neurodegenerative disorders often have reduced BDNF concentrations in blood and brain.
- Nerve growth factor (NGF) is a neurotrophin that induces the survival, development, and function of neurons and has been shown to have direct and indirect effects on immune response.
- Proinflammatory cytokines, which include tumor necrosis factor (TNF)-α, interleukin (IL)-1, IL-6, IL-8
Figure 1. Major neuronal and non-neuronal cell types of the CNS and candidate biomarkers for neuroinflammation, brain injury and neurodegeneration. This schematic depicts possible origins of biomarkers for neuroinflammation, traumatic brain injury, and neurodegeneration. Abbreviations: GFAP (glial fibrillary acidic protein); S100B (S100 calcium-binding protein B); NCAM-1 (neural cell adhesion molecule -1); NF-H (neurofilament heavy polypeptide); UCHL1 (ubiquitin carboxyl-terminal hydrolase isoenzyme L1); TREM-2 (triggering receptor expressed on myeloid cells 2); CHI3L1/YKL-40 (chitinase-3-like protein 1/protein with first three N-terminal amino acids, tyrosine (Y), lysine (K), and leucine (L) with apparent molecular weight of 40); sRAGE (soluble receptor for advanced glycation end products); NGF-beta (nerve growth factor-beta); BDNF (brain-derived neurotrophic factor); GDNF (glial cell-derived neurotrophic factor); CTNF (ciliary neurotrophic factor); BACE1 (beta-secretase 1); KLK6 (kallikrein related peptidase 6); TDP-43 (TAR DNA-binding protein - 43).
Click image to enlarge
Neurobiology ELISA kits
ELISA enables specific, quantitative measurements of protein from a variety of biological sources including serum, plasma, and cerebrospinal fluid (CSF) from a variety of species. We offer ELISA kits for the study of critical proteins such as Aβ40/Aβ42, phosphorylated Tau, and alpha-synuclein implicated in neurodegenerative disorders.
Invitrogen ELISA kits for popular targets such as BDNF, GDNF, Amyloid beta etc. are listed in Table 1.
Standard curve of human Tau (total) and ELISA parallelism between natural and recombinant Human Tau using Tau (Total) Human ELISA Kit is shown in Figure 2.
| NEW! Neurofilament Light (NF-L) ELISA Kit is now available. Discover now |
Learn more about ELISA kits and components
Popular neurobiology protein targets and ELISA performance data
Table 1. View all ELISA kits for the following popular targets:
Figure 2. Performance data for Human Tau (Total) ELISA. ELISA was performed using standard human Tau (total) ranging from 0–2,000 pg/mL and Tau (Total) Human ELISA Kit. Left panel: Typical 7-point standard curve. The dynamic range is 31–2,000 pg/mL, with an analytical sensitivity of <12 pg/mL. Right panel: recombinant human (Hu) tau protein standard provided in the kit was compared to natural human tau and serially diluted in Standard Diluent Buffer. The optical density of each dilution was plotted against the standard curve. Parallelism between the natural and recombinant protein indicates that the standard accurately reflects natural Hu tau content in the samples.
Neurobiology ProQuantum high sensitivity immunoassays
ProQuantum Immunoassay Kits for neurobiology and neuroscience research are designed to provide quantitative measurements of proteins in small sample volumes. Utilizing proximity-based amplification technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly no-wash workflow combined with intuitive software for analytics enables sample-to-answer in just two hours. We continue to launch assays for new targets so check back often to see what is available.
Invitrogen ProQuantum immunoassay kits for popular targets such as Tau, Tau pT181 and Troponin I are listed in Table 2. Standard curve of Tau using Human Tau Total ProQuantum Immunoassay Kit is shown in Figure 3.
Find neurobiology-related ProQuantum assays
Learn more about how the ProQuantum immunoassays work
Read BioProbes Journal article: Introducing ProQuantum High-Sensitivity Immunoassays—The new generation of target-specific protein quantitation
Popular neurobiology research protein targets and ProQuantum assay performance data
Table 2. Neurobiology-related ProQuantum immunoassays. View our ProQuantum immunoassay kits for the following popular targets:
Figure 3. Representative standard curve of Human Tau (Total). The standard curve for human Tau (total) using Human Tau Total ProQuantum Immunoassay Kit shows a large dynamic range (3.2–500,000 pg/mL) of Tau protein.
Neurobiology ProcartaPlex multiplex immunoassays
Invitrogen ProcartaPlex multiplex immunoassay panels provide a powerful biomarker detection tool to help distinguish diseased from non-diseased states and/or even differentiate between neurodegenerative diseases. These Luminex xMAP-based assays allow for the simultaneous measurement and tracking of multiple factors/biomarkers over time and open the avenue of a deeper and more thorough understanding of the progression of neuroinflammation and neurodegenerative diseases. Various biomarker levels were tested in cerebrospinal fluid samples of human adult and pediatric patients using a ProcartaPlex Human Neuroscience Panel (Figure 4).
Select one of our preconfigured panels described below (Table 3) or use the Panel Configurator button to customize your specific panel.
ProcartaPlex Panel Configurator
Learn more about ProcartaPlex multiplex immunoassays
| NEW! Neurofilament Light (NF-L) for Luminex now available. Discover now |
Neurobiology-multiplex immunoassay panels and performance data
Click image to enlarge
Figure 4. Quantification of biomarkers in cerebrospinal fluid and plasma using ProcartaPlex panel. Cerebrospinal fluid samples of 10 ungrouped human adult and 10 human pediatric patients were tested for 18 biomarker levels using a ProcartaPlex Human Neuroscience Panel. Results of the ungrouped human samples are shown for all targets. (Data provided by The Washington University Bursky Center for Human Immunology and Immunotherapy Programs (CHiiPs) Immunomonitoring Laboratory.)
Table 3. Preconfigured ProcartaPlex multiplex immunoassay panels for neurobiology.
| Neuroscience preconfigured multiplex panel | ||
| Product Name | Size | Cat. No. |
ProcartaPlex Human Brain Injury Panel, 5plex Target list [bead region]: | 96 tests | EPX050-15838-901 |
| Neurodegeneration preconfigured multiplex panels | ||
ProcartaPlex Human Neurodegeneration Panel 1, 9plex Target list [bead region]: | 96 tests | EPX090-15836-901 |
ProcartaPlex Human Neurodegeneration Panel 2, 4plex Target list [bead region]: | 96 tests | EPX040-15832-901 |
| Neuroinflammation preconfigured multiplex panel | ||
ProcartaPlex Human Neuroinflammation Panel, 6plex Target list [bead region]: | 96 tests | EPX060-15833-901 |
| Neurotrophic Factor preconfigured multiplex panel | ||
ProcartaPlex Human Neurotrophic Factors Panel, 4plex Target list [bead region]: | 96 tests | EPX040-15828-901 |
Neurobiology QuantiGene multiplex assays
QuantiGene Plex assays offer an accurate and precise method for the detection and quantification of multiple DNA and RNA targets. This assay utilizes branched DNA signal amplification technology to enable the measurement of gene expression levels in a variety of sample types, including blood, tissue, FFPE samples, and cell lysates with no RNA purification required. The ability to simultaneously measure up to 80 targets in a single sample allows researchers to gain a comprehensive view of neurobiological events, helping provide critical insights into the dynamic processes underlying brain development, function, and neurological disorders and diseases.
There are several advantages to bead-based assays over traditional methods of gene expression analysis. QuantiGene Plex supports a highly multiplexed approach, allowing for the simultaneous measurement of multiple targets in a single sample using the same Luminex xMAP technology as ProcartaPlex. This capability not only helps save time and resources but also provides a thorough understanding of gene expression patterns. The assay is robust and highly sensitive, enabling the detection of low-abundance targets with high precision. Without the need for traditional RNA extraction and purification, the lyse-and-go method of QuantiGene Plex helps reduce potential variability and helps save valuable time.
QuantiGene Plex gene expression assays are available in two formats to suit your individual research needs related to neurobiology research. You can choose from a selection of carefully curated preconfigured panels, each targeting a specific set of genes or pathways of interest (Table 5) or use the Panel Configurator to create your own customized panel in 5 easy steps. A few examples of published data using custom QuantiGene plex assays are highlighted in Table 4.
Learn more about QuantiGene multiplex immunoassays
Table 4. List of publications highlighting custom-designed QuantiGene assays for studying neurobiology research.
| Reference | Publication summary |
| Maxi et al., 2022 | Frontal cortex and basal ganglia tissue were used in a custom QuantiGene panel to measure the expression of proinflammatory cytokines and chemokines, anti-inflammatory cytokines, lymphocytes, monocytes and macrophages, microglia-related astrocyte genes in simian immunodeficiency virus-infected macaques. |
| Xu et al., 2022 | In studying bacterial meningitis and the disruption of the blood-brain barrier, a QuantiGene multiplex panel was used to measure cytokine mRNA levels in mouse brains. Specifically, high levels of IL-17A were detected in the brain post-infection, indicating its involvement in regulating the blood-brain barrier. |
| Hicks et al., 2023 | A custom 24-plex panel was used to detect and measure changes in genes associated with Parkinson’s disease. SHSY5Y neuroblastoma cells were treated with siRNA to suppress the histone acetylating non-specific lethal (NSL) complex. |
| Solem et al., 2025 | In researching the neurodegenerative disorder Huntington’s disease, two custom designed QuantiGene panels were used to capture and measure different levels of Htt and mHtt aggregation in the striatum and hippocampus brain regions in mice. |
Table 5. Preconfigured QuantiGene gene expression multiplex immunoassay panels for cell signaling research.
| Product Name | Size (96-well) | Cat. No. |
QuantiGene Plex Human Neuroscience Panel 15-plex Targets: | 1 plate | QGP-115-HUNEUROSCI |
| 3 plates | QGP-215-HUNEUROSCI | |
| 10 plates | QGP-315-HUNEUROSCI | |
QuantiGene Plex Mouse Neuroscience Panel 15-plex Targets: | 1 plate | QGP-115-MSNEUROSCI |
| 3 plates | QGP-215-MSNEUROSCI | |
| 10 plates | QGP-315-MSNEUROSCI | |
QuantiGene Plex Human Neurodegeneration Panel 20-plex Targets: | 1 plate | QGP-120-HUNEURODEG |
| 3 plates | QGP-220-HUNEURODEG | |
| 10 plates | QGP-320-HUNEURODEG | |
QuantiGene Plex Mouse Neurodegeneration Panel 20-plex Targets: | 1 plate | QGP-120-MSNEURODEG |
| 3 plates | QGP-220-MSNEURODEG | |
| 10 plates | QGP-320-MSNEURODEG |
Overview of neurobiology
Quantification of protein biomarkers is critical for neurobiology and neuroscience research and antibody-based techniques, such as enzyme-linked immunosorbent assays (ELISA) and multiplex immunoassays, offer powerful tools to achieve sensitive quantification. These proteins include neurodegenerative disease biomarkers, traumatic brain injury biomarkers, including those released due to astroglial, axonal, and neuronal cell body injury, neurotrophic factors (NTFs) that play a key role in the development of neurons, their plasticity, protection, repair, and key cytokines and chemokines that are involved in central nervous system (CNS) tissue homeostasis and neuroinflammation (Figure 1).
A variety of conditions or diseases of the CNS including brain trauma, stroke, multiple sclerosis, Parkinson’s disease, or Alzheimer’s disease can trigger neuroinflammation and dysfunction of blood-brain barrier (BBB). Acting in concert with aging, epigenetics, the environment, and other risk factors can strongly influence the onset and progression of these diseases. For example, traumatic brain injury has been established as a risk factor for the development of neurodegenerative diseases and dementia at later stages of life. In response to insults that perturb homeostasis in the CNS, a rapid and early activation of the glial cells form the acute inflammatory response. This leads to the repair of the damaged area but if the challenge is not cleared and persists, the activation of the response is exaggerated, becomes chronic, and can result in tissue degeneration and even the drastic loss of BBB integrity.
Key targets for neurodegeneration:
- Amyloid-beta (Aβ, beta amyloid, or Abeta), which is crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer’s patients.
- Tau, a protein which stabilizes microtubules and which, when not functioning properly, is implicated in pathologies and dementias of the nervous system.
- Alpha synuclein (α-synuclein), which is thought to play an important role in maintaining a supply of synaptic vesicles in presynaptic terminals and may also help regulate the release of dopamine.
Key targets for traumatic brain injury:
- Glial fibrillary acidic protein (GFAP) is a monomeric intermediate filament (IF) protein found in the astroglial cytoskeleton. This protein is not found outside the central nervous system and is only released after cell death or injury, when it may be detected in the blood.
- S100B is a calcium-sensor protein that is highly expressed in astrocytes but also present in other cells of the nervous system, including oligodendrocytes, Schwann cells, and ependymal cells. Elevated S100B levels in biological fluids has been shown to be a biomarker for acute brain injury.
- Neurofilament—Heavy (NF-H) and Light (NF-L) are subunits of the neurofilament scaffolding protein of neurons. Neuronal damage can cause the release of neurofilament into the interstitial fluid and subsequently to the cerebrospinal fluid and the blood.
- Ubiquitin C-terminal hydrolase (UCH-L1) is a brain-specific deubiquitinating enzyme. Elevated cerebrospinal fluid and blood concentrations of UCH-L1 have been associated with traumatic brain injury.
Key targets for neuroinflammation:
- Brain-derived neurotrophic factor (BDNF) is a neurotrophin that induces the maintenance, survival, plasticity, and neurotransmitter regulation of neurons, and neurodegenerative disorders often have reduced BDNF concentrations in blood and brain.
- Nerve growth factor (NGF) is a neurotrophin that induces the survival, development, and function of neurons and has been shown to have direct and indirect effects on immune response.
- Proinflammatory cytokines, which include tumor necrosis factor (TNF)-α, interleukin (IL)-1, IL-6, IL-8
Figure 1. Major neuronal and non-neuronal cell types of the CNS and candidate biomarkers for neuroinflammation, brain injury and neurodegeneration. This schematic depicts possible origins of biomarkers for neuroinflammation, traumatic brain injury, and neurodegeneration. Abbreviations: GFAP (glial fibrillary acidic protein); S100B (S100 calcium-binding protein B); NCAM-1 (neural cell adhesion molecule -1); NF-H (neurofilament heavy polypeptide); UCHL1 (ubiquitin carboxyl-terminal hydrolase isoenzyme L1); TREM-2 (triggering receptor expressed on myeloid cells 2); CHI3L1/YKL-40 (chitinase-3-like protein 1/protein with first three N-terminal amino acids, tyrosine (Y), lysine (K), and leucine (L) with apparent molecular weight of 40); sRAGE (soluble receptor for advanced glycation end products); NGF-beta (nerve growth factor-beta); BDNF (brain-derived neurotrophic factor); GDNF (glial cell-derived neurotrophic factor); CTNF (ciliary neurotrophic factor); BACE1 (beta-secretase 1); KLK6 (kallikrein related peptidase 6); TDP-43 (TAR DNA-binding protein - 43).
Click image to enlarge
Neurobiology ELISA kits
ELISA enables specific, quantitative measurements of protein from a variety of biological sources including serum, plasma, and cerebrospinal fluid (CSF) from a variety of species. We offer ELISA kits for the study of critical proteins such as Aβ40/Aβ42, phosphorylated Tau, and alpha-synuclein implicated in neurodegenerative disorders.
Invitrogen ELISA kits for popular targets such as BDNF, GDNF, Amyloid beta etc. are listed in Table 1.
Standard curve of human Tau (total) and ELISA parallelism between natural and recombinant Human Tau using Tau (Total) Human ELISA Kit is shown in Figure 2.
| NEW! Neurofilament Light (NF-L) ELISA Kit is now available. Discover now |
Learn more about ELISA kits and components
Popular neurobiology protein targets and ELISA performance data
Table 1. View all ELISA kits for the following popular targets:
Figure 2. Performance data for Human Tau (Total) ELISA. ELISA was performed using standard human Tau (total) ranging from 0–2,000 pg/mL and Tau (Total) Human ELISA Kit. Left panel: Typical 7-point standard curve. The dynamic range is 31–2,000 pg/mL, with an analytical sensitivity of <12 pg/mL. Right panel: recombinant human (Hu) tau protein standard provided in the kit was compared to natural human tau and serially diluted in Standard Diluent Buffer. The optical density of each dilution was plotted against the standard curve. Parallelism between the natural and recombinant protein indicates that the standard accurately reflects natural Hu tau content in the samples.
Neurobiology ProQuantum high sensitivity immunoassays
ProQuantum Immunoassay Kits for neurobiology and neuroscience research are designed to provide quantitative measurements of proteins in small sample volumes. Utilizing proximity-based amplification technology, the assay combines the analyte specificity of high-affinity antibody-antigen binding with the signal detection and amplification capabilities of real-time PCR to achieve a simple yet powerful next-generation protein quantitation platform. A user-friendly no-wash workflow combined with intuitive software for analytics enables sample-to-answer in just two hours. We continue to launch assays for new targets so check back often to see what is available.
Invitrogen ProQuantum immunoassay kits for popular targets such as Tau, Tau pT181 and Troponin I are listed in Table 2. Standard curve of Tau using Human Tau Total ProQuantum Immunoassay Kit is shown in Figure 3.
Find neurobiology-related ProQuantum assays
Learn more about how the ProQuantum immunoassays work
Read BioProbes Journal article: Introducing ProQuantum High-Sensitivity Immunoassays—The new generation of target-specific protein quantitation
Popular neurobiology research protein targets and ProQuantum assay performance data
Table 2. Neurobiology-related ProQuantum immunoassays. View our ProQuantum immunoassay kits for the following popular targets:
Figure 3. Representative standard curve of Human Tau (Total). The standard curve for human Tau (total) using Human Tau Total ProQuantum Immunoassay Kit shows a large dynamic range (3.2–500,000 pg/mL) of Tau protein.
Neurobiology ProcartaPlex multiplex immunoassays
Invitrogen ProcartaPlex multiplex immunoassay panels provide a powerful biomarker detection tool to help distinguish diseased from non-diseased states and/or even differentiate between neurodegenerative diseases. These Luminex xMAP-based assays allow for the simultaneous measurement and tracking of multiple factors/biomarkers over time and open the avenue of a deeper and more thorough understanding of the progression of neuroinflammation and neurodegenerative diseases. Various biomarker levels were tested in cerebrospinal fluid samples of human adult and pediatric patients using a ProcartaPlex Human Neuroscience Panel (Figure 4).
Select one of our preconfigured panels described below (Table 3) or use the Panel Configurator button to customize your specific panel.
ProcartaPlex Panel Configurator
Learn more about ProcartaPlex multiplex immunoassays
| NEW! Neurofilament Light (NF-L) for Luminex now available. Discover now |
Neurobiology-multiplex immunoassay panels and performance data
Click image to enlarge
Figure 4. Quantification of biomarkers in cerebrospinal fluid and plasma using ProcartaPlex panel. Cerebrospinal fluid samples of 10 ungrouped human adult and 10 human pediatric patients were tested for 18 biomarker levels using a ProcartaPlex Human Neuroscience Panel. Results of the ungrouped human samples are shown for all targets. (Data provided by The Washington University Bursky Center for Human Immunology and Immunotherapy Programs (CHiiPs) Immunomonitoring Laboratory.)
Table 3. Preconfigured ProcartaPlex multiplex immunoassay panels for neurobiology.
| Neuroscience preconfigured multiplex panel | ||
| Product Name | Size | Cat. No. |
ProcartaPlex Human Brain Injury Panel, 5plex Target list [bead region]: | 96 tests | EPX050-15838-901 |
| Neurodegeneration preconfigured multiplex panels | ||
ProcartaPlex Human Neurodegeneration Panel 1, 9plex Target list [bead region]: | 96 tests | EPX090-15836-901 |
ProcartaPlex Human Neurodegeneration Panel 2, 4plex Target list [bead region]: | 96 tests | EPX040-15832-901 |
| Neuroinflammation preconfigured multiplex panel | ||
ProcartaPlex Human Neuroinflammation Panel, 6plex Target list [bead region]: | 96 tests | EPX060-15833-901 |
| Neurotrophic Factor preconfigured multiplex panel | ||
ProcartaPlex Human Neurotrophic Factors Panel, 4plex Target list [bead region]: | 96 tests | EPX040-15828-901 |
Neurobiology QuantiGene multiplex assays
QuantiGene Plex assays offer an accurate and precise method for the detection and quantification of multiple DNA and RNA targets. This assay utilizes branched DNA signal amplification technology to enable the measurement of gene expression levels in a variety of sample types, including blood, tissue, FFPE samples, and cell lysates with no RNA purification required. The ability to simultaneously measure up to 80 targets in a single sample allows researchers to gain a comprehensive view of neurobiological events, helping provide critical insights into the dynamic processes underlying brain development, function, and neurological disorders and diseases.
There are several advantages to bead-based assays over traditional methods of gene expression analysis. QuantiGene Plex supports a highly multiplexed approach, allowing for the simultaneous measurement of multiple targets in a single sample using the same Luminex xMAP technology as ProcartaPlex. This capability not only helps save time and resources but also provides a thorough understanding of gene expression patterns. The assay is robust and highly sensitive, enabling the detection of low-abundance targets with high precision. Without the need for traditional RNA extraction and purification, the lyse-and-go method of QuantiGene Plex helps reduce potential variability and helps save valuable time.
QuantiGene Plex gene expression assays are available in two formats to suit your individual research needs related to neurobiology research. You can choose from a selection of carefully curated preconfigured panels, each targeting a specific set of genes or pathways of interest (Table 5) or use the Panel Configurator to create your own customized panel in 5 easy steps. A few examples of published data using custom QuantiGene plex assays are highlighted in Table 4.
Learn more about QuantiGene multiplex immunoassays
Table 4. List of publications highlighting custom-designed QuantiGene assays for studying neurobiology research.
| Reference | Publication summary |
| Maxi et al., 2022 | Frontal cortex and basal ganglia tissue were used in a custom QuantiGene panel to measure the expression of proinflammatory cytokines and chemokines, anti-inflammatory cytokines, lymphocytes, monocytes and macrophages, microglia-related astrocyte genes in simian immunodeficiency virus-infected macaques. |
| Xu et al., 2022 | In studying bacterial meningitis and the disruption of the blood-brain barrier, a QuantiGene multiplex panel was used to measure cytokine mRNA levels in mouse brains. Specifically, high levels of IL-17A were detected in the brain post-infection, indicating its involvement in regulating the blood-brain barrier. |
| Hicks et al., 2023 | A custom 24-plex panel was used to detect and measure changes in genes associated with Parkinson’s disease. SHSY5Y neuroblastoma cells were treated with siRNA to suppress the histone acetylating non-specific lethal (NSL) complex. |
| Solem et al., 2025 | In researching the neurodegenerative disorder Huntington’s disease, two custom designed QuantiGene panels were used to capture and measure different levels of Htt and mHtt aggregation in the striatum and hippocampus brain regions in mice. |
Table 5. Preconfigured QuantiGene gene expression multiplex immunoassay panels for cell signaling research.
| Product Name | Size (96-well) | Cat. No. |
QuantiGene Plex Human Neuroscience Panel 15-plex Targets: | 1 plate | QGP-115-HUNEUROSCI |
| 3 plates | QGP-215-HUNEUROSCI | |
| 10 plates | QGP-315-HUNEUROSCI | |
QuantiGene Plex Mouse Neuroscience Panel 15-plex Targets: | 1 plate | QGP-115-MSNEUROSCI |
| 3 plates | QGP-215-MSNEUROSCI | |
| 10 plates | QGP-315-MSNEUROSCI | |
QuantiGene Plex Human Neurodegeneration Panel 20-plex Targets: | 1 plate | QGP-120-HUNEURODEG |
| 3 plates | QGP-220-HUNEURODEG | |
| 10 plates | QGP-320-HUNEURODEG | |
QuantiGene Plex Mouse Neurodegeneration Panel 20-plex Targets: | 1 plate | QGP-120-MSNEURODEG |
| 3 plates | QGP-220-MSNEURODEG | |
| 10 plates | QGP-320-MSNEURODEG |
Additional resources for neurobiology immunoassays
Immunoassay instruments
References
- Alterman J.F., Coles A.H., Hall L.M., et al. A High-throughput Assay for mRNA Silencing in Primary Cortical Neurons in vitro with Oligonucleotide Therapeutics. Bio Protoc, 2017. 7(16): e2501.
- Alterman J.F., Godinho B.M.D.C., Hassler M.R., et al. A divalent siRNA chemical scaffold for potent and sustained modulation of gene expression throughout the central nervous system. Nat Biotechnol, 2019. 37(8): 884-894.
- Reisman S.A., Gahir S.S., Lee C.I., et al. Pharmacokinetics and pharmacodynamics of the novel Nrf2 activator omaveloxolone in primates. Drug Des Devel Ther, 2019. 13: 1259-1270.
- Papadopoulou A.S., Gomez-Paredes C., Mason M.A, et al. Extensive Expression Analysis of Htt Transcripts in Brain Regions from the zQ175 HD Mouse Model Using a QuantiGene Multiplex Assay. Sci Rep, 2019. 9(1): 16137.
- Gomez-Paredes C., Mason M.A., Taxy B.A., et al. The heat shock response, determined by QuantiGene multiplex, is impaired in HD mouse models and not caused by HSF1 reduction. Sci Rep, 2021. 11(1): 9117.
- McGahan B.G., Neilsen B.K., Kelly D.L., et al. Assessment of vascularity in glioblastoma and its implications on patient outcomes. J Neurooncol, 2017. 132(1): 35-44.
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