Higher-throughput multiplexing

The Luminex FLEXMAP 3D Instrument System is an advanced and versatile multiplexing platform. A fast read time, compatibility with both 96- and 384-well plates, and ease of integration with front-end automation systems make the Luminex FLEXMAP 3D system well-suited for high-throughput applications. Automated start up, shut down, and maintenance routines make the Luminex FLEXMAP 3D system easy to use and maintain. The system is compatible with Invitrogen ProcartaPlex multiplex immunoassays and Invitrogen QuantiGene Plex multiplex gene expression assays, in both 96- and 384-well format.

 

Features

Key features of the Luminex FLEXMAP 3D Instrument Systems:

Multiplexing

Up to 500 analytes in a single sample

Read time

96-well plate in ~20 min (up to 144,000 tests/hour); 384-well plate in ~75 min (up to 153,600 tests/hour)

Dynamic range (typical)

≥4.5 logs

Daily start-up/shut down

≤30 min

Video gallery

FLEXMAP 3D System Overview
Learn about the FLEXMAP 3D system, Luminex’s most advanced and versatile multiplexing platform. Fast read time, compatibility with both 96- and 384-well plates, and ease of integration with front-end automation systems make the FLEXMAP 3D system
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      1. Now Playing
        Up NextFLEXMAP 3D System Overview

        Learn about the FLEXMAP 3D system, Luminex’s most advanced and versatile multiplexing platform. Fast read time, compatibility with both 96- and 384-well plates, and ease of integration with front-end automation systems make the FLEXMAP 3D system

      2. Now Playing
        Up NextFLEXMAP 3D System Initialization

        Learn how to run the system initialization routine for the FLEXMAP 3D. This is the first task you will run after powering on the instrument and prepares the system for use. The steps include warming the laser, checking for fluidics carryover,

      3. Now Playing
        Up NextFLEXMAP 3D Probe Height Adjustment for 384-well Plates

        Correct sample probe height is critical to successful calibration and sample acquisition. This video demonstrates the correct use of probe height adjustment tool when calibrating the probe height for a 384-well mylar or filter bottom plates

      4. Now Playing
        Up NextFLEXMAP 3D System Shutdown Procedure

        In this video, you will learn how to run the shutdown routine on the FLEXMAP 3D system. The shutdown routine will clean and flush fluidics, prevent clogs and crystallization of salt in the fluidics and/or sample probe

      5. Now Playing
        Up NextFLEXMAP 3D Cleaning the Sample Probe

        Watch this video to recognize clogged probe indicators with your FLEXMAP 3D instrument. Learn about how to remove the clogged probe, clean the probe, reinstall and recalibrate the instrument to ensure proper acquisitions.

      6. Now Playing
        Up NextFLEXMAP 3D Clog Removal

        Learn how to perform a clog removal routine on your FLEXMAP 3D system to prevent clogs in the fluid lines and sample probe.

    Specifications

     

    FLEXMAP 3D System

    Optics

    Lasers, APDs, PMTs

    Hardware

    Flow cytometry–based lasers, APDs, PMTs

    Footprint including PC (linear bench space)

    110 cm x 62 cm x 63 cm

    Weight (analyzer)

    77.1 kg (170 lb)

    Multiplex capacity

    Up to 500

    Maximum available commercial assay plex

    80

    Application

    Protein/nucleic acid

    Sample volume

    6.3–80 µL (assay dependent) 

    Plate format 

    96-well or 384-well

    Read time

    		 96-well in ~20 min
    384-well in ~75 min

    Sensitivity (assay dependent)

    0.06–1 pg/mL (immunoassays)
    1,000 RNA copies per target/well (RNA assays)

    Reporter laser/optics

    532 nm (green) 

    Dynamic range

    ≥4.5 logs (RP1)

    Operational validation

    Calibration, verification, and fluidics

    21 CFR compliance

    Yes

    Automation

    Integration software

    Analytics (software)

    xPONENT basic plus Thermo Fisher Connect Platform

    For Research Use Only. Not for use in diagnostic procedures.