Make way for more powerful imaging

The fully automated EVOS M7000 Imaging System was designed to simplify the most demanding slide and cell-based imaging applications including live-cell analysis, image tiling, and Z-stacking. It is equipped with advanced 2D deconvolution capabilities which increase signal-to-noise ratio (SNR) and provide clean, accurate information, allowing you to reveal hidden insights within your samples.

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    Features

    In addition to the common features of all EVOS microscopes, the EVOS M7000 Imaging System offers:

    Full automation

    Automated routines help streamline workflow and improve experimental reproducibility
     

    Speed

    Scan a 96-well plate in 3 fluorescence channels in less than 5 minutes

     

    2D deconvolution

    Capture images with exceptional clarity and detail

    Time-lapse live-cell imaging

    Optional onstage incubator enables precise control over temperature, humidity, and gas levels
     

    Area view

    Move rapidly and seamlessly between single-field mode and low- and high-magnification scan modes to easily define and capture the area of interest
     

    Data analysis

    Seamlessly transfer images to optional
    Celleste Image Analysis Software for access to powerful tools for image segmentation, classification, and cell-based assays

    Virtual 360º view

    Check out the EVOS M7000 Imaging System virtually.

     

    Explore 360º view

     

    Note: This tool is best viewed in Chrome, Firefox, or Safari browser.

    Learn more

    EVOS_M7000 instrument

    Advanced 2D deconvolution

    Experience the exceptional clarity and detail of 2D deconvolution.

    Two images side-by-side comparing mouse kidney section before (left) and after (right) deconvolution.

    FluoCells™ Prepared Slide #3 (mouse kidney section with Alexa Fluor™ 488 WGA, Alexa Fluor™ 568 Phalloidin, and DAPI), imaged at 4X (Cat. No. AMEP4922) using EVOS M7000 Imaging System, deconvolved using EVOS Analysis.

    Two images side-by-side comparing U2OS cells before (left) and after (right) deconvolution.

    U2OS cells plated in Nunc™ Glass Bottom Dishes (Cat. No. 150682) and labeled with Hoechst 33342, trihydrochloride trihydrate, 10 mg/mL (Cat. No. H3570, nucleus-blue), Alexa Fluor™ 488 phalloidin (Cat. No. A12379, actin-green), and alpha Tubulin Monoclonal Antibody (236-10501) (Cat. No. A11126) with Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647 (Cat. No. A21235, tubulin-purple).  Imaged on EVOS M7000 Imaging System with Olympus 100x Objective, semi-apo, 0.95 NA/0.2WD (Cat. No. AMEP4988).

    Two images side-by-side comparing Hela cells before (left) and after (right) deconvolution.

    Hela cells, PFA fixed and stained with DAPI, anti-coilin (GFP), anti-tubulin (Cy5), and Ki-67 (RFP), imaged using EVOS M7000 Imaging System with 20x fluorite objective (Cat. No. AMEP4698), deconvolved using EVOS Analysis software.

    EVOS M7000 HCA Package

    The EVOS M7000 Imaging System, High-Content Analysis Package (AMF7000HCA) combines the fully automated, inverted, multi-channel fluorescence and transmitted light imaging system with Celleste 6 Image Analysis Software, a full-featured image analysis suite. The Celleste 6 Image Analysis software facilitates the transition into low-throughput high-content analyses (HCA) while maintaining the versatility and power of the EVOS M7000 automated microscope. 
     

    Using plate-based multi-channel analysis (MCA) protocols leveraging machine-learning based algorithms together with its icon-based wizard-driven workflow, Celleste 6 software can help you efficiently segment and classify images for a wide range of assays or bioapplications, including neurite outgrowth, angiogenesis, cell viability, transfection efficiency, and many more. The software also offers a wide range of well-plate data displays including heat maps, image montages, and kinetic graphing options.

    EVOS M7000 & Celleste 6 HCA Packages

    Description

    Cat. No

    EVOS M7000 Imaging System, High Content Analysis Package

    • EVOS M7000
    • Celleste 6 Image Analysis Software, Single-user License

    AMF7000HCA

    EVOS M7000 Imaging System, High Content Analysis Package with 3D

    • EVOS M7000
    • Celleste 6 Image Analysis Software, Single-user License
    • Celleste 3D Deconvolution Software, Single-user License
    • Celleste GPU Acceleration Software for 3D Deconvolution, Single-user License
    • Celleste 3D Visualization Software, Single-user License
    • Celleste 3D Analysis Software, Single-user License

    AMF7000HCA3D
    Image shows various bio-applications available with multi-class feature of Celleste

    Featured images and videos

    1:59

    EVOS M7000- Automated, safe, live-cell imaging, tiling and image stitching

    Explore the automated, safe, live or dead cell imaging and advanced image processing like tile-stitching with EVOS M7000, visit: thermofisher.com/evosm7000
    2:20

    EVOS M7000- Z-stacking, 2D/3D- deconvolution, visualization and analysis

    Explore the Z-stacking features with M7000 and 2D/3D deconvolution and analysis with Celleste Software, visit: thermofisher.com/evosm7000
    2:05

    EVOS M7000- Optimized light cubes and software features

    Explore the high-class imagery achievable with our optimized light cubes and new software features, visit: thermofisher.com/evosm7000

    HeLa cell widefield vs deconvolved

    EVOS M7000, 60x objective, Celleste Image Analysis Software. Stains: NucBlue (nucleus), Phalloidin (F-actin).

    Image displays ability of EVOS M5000 to capture multicolor fluorescent images

    Stitching of rat brain tissue

    EVOS M7000, 4X objective, 16 x 9 stitched images.

    Image displays ability of EVOS M5000 to capture multicolor fluorescent images

    A549 Spheroid

    EVOS M7000, 10X objective. Stains: NucBlue, MitoTracker Orange, CellROX Deep Red.

    Image displays ability of EVOS M5000 to capture multicolor fluorescent images

    Proliferating cells in rat intestine section

    EVOS M7000, color camera.

    Image displays ability of EVOS M5000 to capture multicolor fluorescent images

    Human Cardiac Arterial Smooth Muscle (HCASM) primary cells

    EVOS M5000. 10x objective.

    Image displays ability of EVOS M5000 to capture multicolor fluorescent images
    0:05

    Wound healing time-lapse movie

    EVOS M7000, 10x, Onstage Incubator over 21 hours. Stains: DsRed-cadherin-4, GFP. (0:17)
    0:05

    Z-stack movie

    A series of images were captured on the EVOS FL Auto Cell Imaging System. Creating a Z-stack from these images allowed the observation of cellular cytoskeletal changes, which can be indicative of the loss of cell health.
    0:00

    HepG2 spheroid 3D deconvolution and animation

    EVOS M7000, 10x, Celleste Image Analysis Software. Stains: Hoechst (blue), Phalloidin (red). (0:20)

    View more M7000 images and movies in our Image Gallery and Video Gallery.
     

    Enhance your imaging experiments with optimized product pairings

    Save time with carefully matched product pairings designed to deliver exceptional imaging results.

    Specifications

    Category Attribute Description
    Optics Description Infinity-corrected optical system; RMS threaded objectives with 45 mm parfocal distance
    Imaging mode Fluorescence, brightfield, color brightfield, phase contrast
    Imaging methods Single color, multicolor, area scan with montage or tile-stitch, time lapse, Z-stacking, movie capture
    Illumination Adjustable-intensity LED light cubes with integrated hard-coated filter set and >50,000-hour life
    Light cube capacity 5-position chamber for 4 fluorescence cubes + brightfield
    Light cubes (not included)

    Broad selection of standard and specialty light cubes. Commonly used light cubes include (ex/em):

    • DAPI (357/447 nm)

    • GFP (470/525 nm)

    • RFP (531/593 nm)

    • Texas Red (585/624 nm)

    • Cy5 (628/692 nm)
    Objective capacity 5-position automated turret
    Objectives (not included) Wide selection of high-quality long working distance (LWD) and coverslip-corrected objectives
    Condenser 60-mm LWD condenser; 4-position turret with a clear aperture and 3 phase annuli
    Focus mechanism Automated focus with sub-micron (0.150 µm) resolution (single-step accuracy)
    Monochrome camera High-sensitivity 3.2 MP (2048 x 1536) CMOS sensor with 3.45 µm pixel resolution
    Color camera High-sensitivity 3.2 MP (2048 x 1536) CMOS sensor with 3.45 µm pixel resolution
    Captured images

    16-bit RAW monochrome: TIFF, PNG (12-bit dynamic range)

    8-bit color: TIFF, PNG, JPG

    Movies and time-lapse: AVI, WMV
    Physical characteristics Power supply 24V AC adapter with country-specific power cords
    Dimensions (W x H x D)

    18 x 14 x 13 inches

    45.7 x 33.0 x 35.6 cm
    Weight 26 lbs (11.8 kg)
    X/Y scanning stage

    Travel range 120 mm x 80 mm with sub-micron resolution

    Drop-in inserts to receive vessel holders and lock-down holders to fix sample in place during long scans
    Stage mechanism Motorized
    Automation Plate scanning Automated multiwell
    System Computer External Dell XE4 computer with Intel Core i9 processor, 128 GB DDR4 RAM, 2 TB SSD, and NVIDIA Quadro A4000 with 8 GB discrete video graphics running Windows®10 21H2, LTSC
    Output ports Computer: 1 x USB 3.1 Gen 2 Type-C; 5 x USB 3.1 Gen 1 Type-A; 4 x USB 2.0 Type-A; 1 Serial; 2 Display Ports 1.2; 1 RJ-45; 2 PS/2; 1 UAJ; 1 Line-out
    Networking capability Connection through Windows/SMB network via Ethernet cable connection

    For Research Use Only. Not for use in diagnostic procedures.