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Estrogen Receptor Antibody (PA5-16476) in IF
Immunofluorescent analysis of Estrogen Receptor (ER, green) in T47D and Hs 578T breast cancer cells. The cells were fixed with 4% paraformaldehyde for 15 min at room temp, permeabilized with 0.1% Triton X-100 for 15 min at room temp, and blocked with 0.3% BSA in PBS for at least 30 min at room temp. Cells were stained with an ER polyclonal antibody (Product ## PA5-16476) at a dilution of 1:100 in blocking buffer overnight at 4C, and then incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody (Product ## 35552) at a dilution of 1:500 for at least 1 hour at room temp. Nuclei (blue) were stained with DAPI (Product ## 62247). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
