Group product Image of a bag of Detachable Dynabeads release buffer and vial of Detachable Dynabeads CD3/CD28

Gibco CTS Detachable Dynabeads beads possess a proprietary active release mechanism—the first of its kind for GMP, clinical trial, and commercial manufacturing use. Gibco CTS Dynabeads magnetic beads are a trusted technology for the isolation, activation, and/or expansion of T cells ex vivo. Whether working with polyclonal T cells, antigen-specific T cells, gene-modified T cells, regulatory T cells, or other T cell subtypes, CTS Dynabeads magnetic beads enable the recovery of T cells with properties comparable to in vivo–activated T cells.

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Gibco CTS Detachable Dynabeads are designed to work with the CTS DynaCellect System to enhance cell therapy manufacturing by ensuring optimal T cell isolation, activation, and purity.

Key Features

  • System compatibility: Designed for use with the CTS DynaCellect System
  • Flexibility and scalability: Suitable for various manufacturing processes
  • Consistent performance: Supports consistent isolation, activation, and purity of T cell populations
  • Advanced technology: Uses paramagnetic beads with VHH antibodies targeting CD markers
  • Release mechanism: Cells are detached using the Gibco CTS Detachable Dynabeads Release Buffer through a competitive binding mechanism
  • Optimal outcomes: Designed to achieve high yield, desired phenotype, and cell viability of T cell subpopulations
     

CTS Detachable Dynabeads CD4 and CD8

Whether you are isolating CD4+ or CD8+ T cell subtypes separately or in combination, you now can actively release the beads from target cells immediately after isolation using Gibco CTS Detachable Dynabeads CD4 and Gibco CTS Detachable Dynabeads CD8 products. No matter the workflow, when used with the Gibco CTS Detachable Dynabeads Release Buffer, both offerings are designed for increased control over target cell isolation and bead removal steps, compatibility with downstream genetic modification, high levels of pure T cells free from ancillary material, and optimal target cell recovery for clinical trials or commercial manufacturing.

Figure 1. CD4+ and CD8+ T cell purity using the CTS Detachable Dynabeads CD4 and CD8 products with the CTS DynaCellect Magnetic Separation System.
Left panel:
The variable starting material of three healthy donors (donors G, H, and I) contained an average 65% frequency of target T cells.
Right panel: The combined isolations achieved a high average purity of 99% of the target T cells.


Ordering information

CTS Detachable Dynabeads CD3/CD28

Gibco CTS Detachable Dynabeads CD3/CD28 magnetic beads are a flexible and scalable platform technology that helps enable isolation and activation in one step while possessing a differentiating active release mechanism. CTS Detachable Dynabeads CD3/CD28 has been found to be comparable to the passive detachable Dynabeads in IL-2 expression, activation marker profile, cytokine release panel, and cell proliferation levels. The key difference is the added release functionality to address biological variability and helps provide users with flexibility in their manufacturing process.

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    Figure 2. CTS Detachable Dynabeads CD3/CD28 induces similar expression of activation markers for A) CD69 and B) CD25 as the passive release CTS Dynabeads CD3/CD28. On days 2 and 3 post-activation, 94% to 98% of T cells are expressing the CD25 activation marker, respectively.​


    Ordering information

    CTS Dynabeads CD3/CD28

    Used in more than 100 clinical trials, Gibco CTS Dynabeads magnetic beads are a trusted technology for the isolation, activation, and/or expansion of T cells ex vivo. Whether working with polyclonal T cells, antigen-specific T cells, gene-modified T cells, regulatory T cells, or other T cell subtypes, CTS Dynabeads magnetic beads enable the recovery of T cells with properties comparable to in vivo–activated T cells. This one-step process of simultaneous T cell isolation and activation yields pure and uniformly activated T cells.

    Figure 3. Activated T cells typically expand >100-fold in 10 days. (A, B) Isolated and activated T cells were expanded for up to 10 days, and both CD4+ and CD8+ T cells proliferated. (C, D) On day 10 post-activation, there were mainly CD45RA+/– CD127+/– CD28+ CD62L+ cells with low PD-1 expression.


    Ordering information

    Catalog # Name Size Price (USD) Qty
    40203D CTS™ (Cell Therapy Systems) Dynabeads™ CD3/CD28 Each
    7,436.60

    CTS Treg Xpander

    Gibco CTS Dynabeads Treg Xpander provides an optimized activation signal for activation and expansion of Regulatory T cells (Tregs) and yields high-fold expansion of suppressive Tregs with a stable Treg phenotype, which is essential for use of Tregs in clinical applications.

    Key features

    • Scalable technology for robust activation and expansion of Tregs
    • Clinically relevant numbers of Tregs with >800-fold expansion after two stimulations
    • Aseptically manufactured and ready for clinical trials and commercial manufacturing
    • Maintained Treg phenotype (FoxP3) and suppressive function
    • Drug Master File available
    • Conformity with cGMP guidelines

    Ordering information

    Catalog # Name Size Price (USD) Qty
    46000D CTS™ Dynabeads™ Treg Xpander Each
    7,457.20

    Dynabeads Human T-Expander CD3/CD28 

    Dynabeads Human T-Expander CD3/CD28, the research-grade version of CTS Dynabead CD3/CD28, is intended for separation, activation, and expansion of human T cells. Containing the same amount and ratio of antibodies from the same clones as CTS Dynabeads CD3/CD28, Dynabeads Human T-Expander CD3/CD28 are intended for small-scale pre-clinical research. Dynabeads Human T-Expander CD3/CD28 offers a simple method for the activation and expansion of T cells that does not require antigen presenting cells or antigen.


    Ordering information

    Catalog # Name Size Price (USD) Qty
    11141D Dynabeads™ Human T-Expander CD3/CD28 Each
    2,755.25

    CTS Detachable Dynabeads CD4 and CD8

    Whether you are isolating CD4+ or CD8+ T cell subtypes separately or in combination, you now can actively release the beads from target cells immediately after isolation using Gibco CTS Detachable Dynabeads CD4 and Gibco CTS Detachable Dynabeads CD8 products. No matter the workflow, when used with the Gibco CTS Detachable Dynabeads Release Buffer, both offerings are designed for increased control over target cell isolation and bead removal steps, compatibility with downstream genetic modification, high levels of pure T cells free from ancillary material, and optimal target cell recovery for clinical trials or commercial manufacturing.

    Figure 1. CD4+ and CD8+ T cell purity using the CTS Detachable Dynabeads CD4 and CD8 products with the CTS DynaCellect Magnetic Separation System.
    Left panel:
    The variable starting material of three healthy donors (donors G, H, and I) contained an average 65% frequency of target T cells.
    Right panel: The combined isolations achieved a high average purity of 99% of the target T cells.


    Ordering information

    CTS Detachable Dynabeads CD3/CD28

    Gibco CTS Detachable Dynabeads CD3/CD28 magnetic beads are a flexible and scalable platform technology that helps enable isolation and activation in one step while possessing a differentiating active release mechanism. CTS Detachable Dynabeads CD3/CD28 has been found to be comparable to the passive detachable Dynabeads in IL-2 expression, activation marker profile, cytokine release panel, and cell proliferation levels. The key difference is the added release functionality to address biological variability and helps provide users with flexibility in their manufacturing process.

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      Figure 2. CTS Detachable Dynabeads CD3/CD28 induces similar expression of activation markers for A) CD69 and B) CD25 as the passive release CTS Dynabeads CD3/CD28. On days 2 and 3 post-activation, 94% to 98% of T cells are expressing the CD25 activation marker, respectively.​


      Ordering information

      CTS Dynabeads CD3/CD28

      Used in more than 100 clinical trials, Gibco CTS Dynabeads magnetic beads are a trusted technology for the isolation, activation, and/or expansion of T cells ex vivo. Whether working with polyclonal T cells, antigen-specific T cells, gene-modified T cells, regulatory T cells, or other T cell subtypes, CTS Dynabeads magnetic beads enable the recovery of T cells with properties comparable to in vivo–activated T cells. This one-step process of simultaneous T cell isolation and activation yields pure and uniformly activated T cells.

      Figure 3. Activated T cells typically expand >100-fold in 10 days. (A, B) Isolated and activated T cells were expanded for up to 10 days, and both CD4+ and CD8+ T cells proliferated. (C, D) On day 10 post-activation, there were mainly CD45RA+/– CD127+/– CD28+ CD62L+ cells with low PD-1 expression.


      Ordering information

      Catalog # Name Size Price (USD) Qty
      40203D CTS™ (Cell Therapy Systems) Dynabeads™ CD3/CD28 Each
      7,436.60

      CTS Treg Xpander

      Gibco CTS Dynabeads Treg Xpander provides an optimized activation signal for activation and expansion of Regulatory T cells (Tregs) and yields high-fold expansion of suppressive Tregs with a stable Treg phenotype, which is essential for use of Tregs in clinical applications.

      Key features

      • Scalable technology for robust activation and expansion of Tregs
      • Clinically relevant numbers of Tregs with >800-fold expansion after two stimulations
      • Aseptically manufactured and ready for clinical trials and commercial manufacturing
      • Maintained Treg phenotype (FoxP3) and suppressive function
      • Drug Master File available
      • Conformity with cGMP guidelines

      Ordering information

      Catalog # Name Size Price (USD) Qty
      46000D CTS™ Dynabeads™ Treg Xpander Each
      7,457.20

      Dynabeads Human T-Expander CD3/CD28 

      Dynabeads Human T-Expander CD3/CD28, the research-grade version of CTS Dynabead CD3/CD28, is intended for separation, activation, and expansion of human T cells. Containing the same amount and ratio of antibodies from the same clones as CTS Dynabeads CD3/CD28, Dynabeads Human T-Expander CD3/CD28 are intended for small-scale pre-clinical research. Dynabeads Human T-Expander CD3/CD28 offers a simple method for the activation and expansion of T cells that does not require antigen presenting cells or antigen.


      Ordering information

      Catalog # Name Size Price (USD) Qty
      11141D Dynabeads™ Human T-Expander CD3/CD28 Each
      2,755.25

      Bioprocessing resources

      Access a targeted collection of scientific application notes, case studies, posters, white papers and more for bioprocessing:

      Intended use of the products mentioned on this page vary. For specific intended use statements please refer to the product label.