Resources for the Countess II Automated Cell Counter

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Documentation and literature

Customer stories

Software updates

Educational resources

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Support for Countess II Automated Cell Counters: online and downloadable materials

The manuals, brochures, application notes, downloads, demos, videos, and other resources in this section will help you get the most out of your Countess II Automated Cell Counter.

Documentation and literature

Countess II

User Guide: Countess II Automated Cell Counter

User Guide: Countess II Automated Cell Counter (ES)

User Guide: Countess II Automated Cell Counter (FR)

Countess II Quick Reference Guide

Countess II FL

User Guide: Countess II FL Automated Cell Counter

User Guide: Countess II FL Automated Cell Counter (ES)

User Guide: Countess II FL Automated Cell Counter (FR)

Countess II FL Quick Reference Guide

Best practices

The four pillars of achieving accurate and precise cell counts

Flow cytometry/FACS

The importance of accurate cell counting in flow cytometry and cell sorting

13-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows for the identification of B cells, NK cells, multiple T cell subsets and myeloid cells

Automated vs manual cell counting

Comparison of cell counting using Countess II Automated Cell Counters vs. hemocytometers

Viability assays

Fluorescent viability assays on the Countess II FL Automated Cell Counter

Incorporate automation into your cell analysis workflows (BioProbes 72)

Apoptosis assays

Fluorescent apoptosis evaluation using the Countess II FL Automated Cell Counter

Transfection/transduction efficiency

Fluorescent protein reporter gene transduction efficiency measured with the Countess II FL Automated Cell Counter

PBMC counting and viability

Blood cell counting using the Countess II FL Automated Cell Counter

Genome editing

Optimizing CRISPR-Cas9 lentiviral transduction efficiency and antibiotic selection measurements

CAR-T cell culture QC

Cell culture quality control during T cell expansion

Adipogenesis

Simple and accurate monitoring of adipogenesis

Customer stories

“Fast count and nice instrument, easy to use!! I love the small counter!”

Dr. Xin Xu, Baylor College of Medicine

“It’s very easy to use and it also allows me to quickly update my lab notebook so that each experiment uses the same number of cells. Quick and efficient.”

Lakeisha Tillery, Meharry Medical College

“It outperformed all other competitors' models that we tried.”

Michael DaCosta, The Jackson Laboratory

“It is very effective at counting cells. It works very well.”

Mairead Bretney, Sanford Burnham Medical Research Institute

“The Countess II makes it very easy to count cells accurately and quickly—the fact that it tells you the dead cell and live cell count is icing on the cake.”

Faryal Mir, MIT

“We compared to a hemocytometer and the Countess II always obtained very close results. The fluorescent measurements are a great tool for us to quantify the % transfection and transduction efficiency in our experiments. The light cubes used to detect fluorescence are very easy to switch out when more than two fluorescence sources are needing to be detected. The automatic focusing is very effective and accurate with the option to manually focus.”

David Waynar, Santa Cruz Biotechnology

“This equipment is very useful for the purposes it is used in our lab. We love it!”

Kristin Harwood, BioMarker Strategies, LLC

“Great for a quick check of transfection/transduction efficiency. Easy to use and seems pretty sensitive.”

Alex Peister, Morehouse College

“Great device. Broad range of cell densities that it can handle. Good at distinguishing clumps and counting cells within clumps.”

Joanne Rzepka, Veterinary Medical Research & Development

“Low maintenance, fast easy accurate cell counts. Easy for students to use and comprehend.”

Robert Dean, D’Youville College

“A very accurate cell counter and you can use it to count fluorescent cells as well. Excellent product. I’m very happy with it. I highly recommend it.”

Massimo Ammirante, University of California, San Diego

Videos

Updating software on the Countess II FL Automated Cell Counter

When the Countess II and Countess II FL Automated Cell Counters require a software update, the process is simple. Just watch this quick video for the easy steps to update.

Automated cell counter – reusable slide, two optional fluorescent channels

Discover how this cell counter can simplify your workflow.

Performing a simple brightfield count on the Countess II FL Automated Cell Counter

Count cells, measure cell viability, and monitor fluorescent protein expression—in just 10 seconds with no slides or tips to buy.

Using the reusable hemocytometer on the Countess II FL Automated Cell Counter

See how to load sample into the economical Countess II Reusable Slide, place it in the slide holder, and insert it into the Countess II FL Automated Cell Counter.

Changing the EVOS Light Cubes on the Countess II FL Automated Cell Counter

See how easy it is to remove one interchangeable EVOS light cube from your Countess II FL Automated Cell Counter and insert another.

Software updates

To download the latest software for Countess II Automated Cell Counters, see our Software Download page. Then view the software update how-to video in the Videos section of this page.

Check for software updates

Educational resources

Educational resources for Countess Automated Cell Counters include an abundance of materials on cell imaging, cell counting, and cell culture. For training courses taught at your site and online, see Services.

Cell Analysis Learning Center

The learning center contains resources for cell analysis techniques and basic principles. Subtopics relevant to Countess II products include Cell counting information and the Molecular Probes School of Fluorescence.

Enter the learning center

Cell Analysis Learning Center s002380

Gibco Cell Culture Basics

Our free introduction to cell culture, covering topics such as laboratory setup, safety, and aseptic technique. You’ll also find basic cell culture techniques and methods for passaging, freezing, and thawing cultured cells.

A single cell depicted in blue

Light cube options

Compatible fluorescent dyes

Verified cell lines

Cell lines verified on the Countess II Automated Cell Counters

Cell type	Organism	Tissue/organ source	Cell size (diameter)
A431	Human	Skin	15.5 μm
Adipocytes	Human	Adipose-derived stem cells	13 μm
Aortic smooth muscle	Human	Smooth muscle	20 μm
Blood, whole lysed	Human	Blood	NA
CHO-M1WT2	Chinese hamster	Ovary	NA
CHSE	Chinook salmon	Embryo	16–17 μm
COLO-205	Human	Colon	NA
COS-7 A	Human	Kidney	NA
HEK293	Human	Kidney	13 μm
HeLa	Human	Cervix	NA
HepG2	Human	Liver	18 μm
HL-60	Human	Blood	NA
J774A.1	Mouse	Blood	13–14 μm
Jurkat	Human	Blood	12 μm
K562	Human	Bone marrow	NA
MCF-7	Human	Breast	20–24 μm
MRC-5	Human	Lung	18 μm
NIH/3T3	Mouse	Embryo	18 μm
PBMC	Human	Human	7–8 μm
PC-12	Rat	Adrenal gland	12–14 μm
Pulmonary artery endothelial cells	Human	Blood vessel	13 μm
Pulmonary artery smooth muscle	Human	Smooth muscle	20 μm
SF-21	Insect	Ovary	NA
U266	Human	Blood	12–13 μm
U2OS	Human	Bone	NA
Umbilical vein endothelial cells	Human	Blood vessel	17 μm

Citations

Shi B et al. (2018). Inhibition of HIV early replication by the p53 and its downstream gene p21. Virology journal 15(1) 53.

Sykora P et al. (2018). Next generation high throughput DNA damage detection platform for genotoxic compound screening. Scientific reports 8(1) 2771.

Elango J et al. (2018). Cross-talk between primary osteocytes and bone marrow macrophages for osteoclastogenesis upon collagen treatment. Scientific reports 8(1) 5318.

Poh A R et al. (2017). Inhibition of hematopoietic cell kinase activity suppresses myeloid cell-mediated colon cancer progression. Cancer cell 31(4) 563–575.

Feng Y et al. (2016) Ratiometric electrochemiluminescence detection of circulating tumor cells and cell-surface glycans. J Electroanal Chem 25 July 2016.

Pirhaji L et al. (2016) Revealing disease-associated pathways by network integration of untargeted metabolomics. Nat Methods 13(9):770–776.doi:10.1038/nmeth.3940.

Delgado RN et al. (2016) Maintenance of neural stem cell regional identity in culture. Neurogenesis 3(1).

Murtaza M et al. (2016) Rotenone susceptibility phenotype in olfactory derived patient cells as a model of idiopathic Parkinson’s Disease. PLoS One 11(4):e0154544. doi:10.1371/journal.pone.0154544.

Vuković LD et al. (2016) Nuclear size is sensitive to NTF2 protein levels in a manner dependent on Ran binding. J Cell Sci 129(6):1115–1127.

Jang DH et al. (2016) Impairment of mitochondrial respiration following ex vivo cyanide exposure in peripheral blood mononuclear cells. Clin Toxicol (Phila) 54(4):303–307.

Jiang X et al. (2016) Protective effect of C4a against hyperoxic lung injury via a macrophage‑dependent but not a neutrophil/lymphocyte‑dependent signaling pathway. Mol Med Rep 13(2):1250–1256.

Wu R et al. (2015) Fabrication and evaluation of multilayer nanofiber-hydrogel meshes with a controlled release property. Fibers 3(3):296–308.

Labedz-Maslowska A et al. (2015) Monocyte chemoattractant protein-induced protein 1 (MCPIP1) enhances angiogenic and cardiomyogenic potential of murine bone marrow-derived mesenchymal stem cells. PLoS One 10(7):e0133746.

Huynh LM et al. (2015) Two histone variants TH2A and TH2B enhance human induced pluripotent stem cell generation. Stem Cells Dev 25(3):251–258.

Takahashi T (2018). Applicability of automated cell counter with a chlorophyll detector in routine management of microalgae. Scientific reports 8(1) 4967.

Jimenez A G et al. (2018) Effects of short‐term clomipramine on anxiety‐like behavior cellular metabolism and oxidative stress in primary fibroblast cells of male and female rats. Physiological reports 6(9) e13615.

Dyavar S R et al. (2018). Normalization of cell associated antiretroviral drug concentrations with a novel RPP30 droplet digital PCR assay. Scientific reports 8(1) 3626.

Li H et al. (2018). Antitumor activity of EGFR-specific CAR T cells against non-small-cell lung cancer cells in vitro and in mice. Cell death & disease 9(2) 177.

Dong L et al. (2018). Verteporfin inhibits YAP-induced bladder cancer cell growth and invasion via Hippo signaling pathway. International journal of medical sciences 15(6) 645.

DeRosa B A et al. (2018). Convergent pathways in idiopathic autism revealed by time-course transcriptomic analysis of patient-derived neurons. Scientific Reports 8(1) 8423.

Akatsuka A et al. (2016 ) A novel thiophene‐3‐carboxamide analog of annonaceous acetogenin exhibits antitumor activity via inhibition of mitochondrial complex I. Pharmacol Res Perspect 4(4)

Meng XQ et al. (2016) Solanine-induced reactive oxygen species inhibit the growth of human hepatocellular carcinoma HepG2 cells. Oncol Lett 11(3):2145–2151

Huang CH et al. (2016) Paper-based microreactor array for rapid screening of cell signaling cascades. Lab Chip 16(15):2911–2920

Swioklo S et al. (2016) Alginate-encapsulation for the improved hypothermic preservation of human adipose-derived stem cells. Stem Cells Transl Med 5(3):339–349.

Huang D et al. (2016) Rapid separation of human breast cancer cells from blood using a simple spiral channel device. Anal Methods 8(30):5940–5948.

Choudhury B et al. (2016) Anticancer activity of Garcinia morella on T-cell murine lymphoma via apoptotic induction. Front Pharmacol 7:3

Yang H et al. (2016) Effect of a feeder layer composed of mouse embryonic and human foreskin fibroblasts on the proliferation of human embryonic stem cells. Exp Ther Med 11(6):2321–2328.

MacLean JA II et al. (2016) WNT7A regulation by miR-15b in ovarian cancer. PLoS One 11(5):e0156109. doi:10.1371/journal.pone.0156109.

Wang J et al. (2016) Investigation of the anti-asthmatic activity of Oridonin on a mouse model of asthma. Mol Med Rep 14(3):2000–2006.

Deleon H (2016) Application of polystyrene disk substrates in cellular cultivation methods: generalized specimen preparation protocol for scanning electron microscopy. J Microsc May 30. doi:10.1111/jmi.12429.

Hasegawa M et al. (2016) Surface coating of a metal mesh device sensor with gold to improve the separation and sensing of mammalian cells. IEEE Sens J 16(13):5129–5135.

Ok SH et al. (2016) Lipid emulsion attenuates apoptosis induced by a toxic dose of bupivacaine in H9c2 rat cardiomyoblast cells. Human Exp Toxicol 35(9):929–937. doi:10.1177/0960327115608930.

Li K et al. (2015) Clinically applicable magnetic-labeling of natural killer cells for MRI of transcatheter delivery to liver tumors: preclinical validation for clinical translation. Nanomedicine (Lond) 10(11):1761–1774.

Roma A et al. (2015) Selective induction of apoptosis by Azadirachta indica leaf extract by targeting oxidative vulnerabilities in human cancer cells. J Pharm Pharm Sci 18(4):729–746.

Malvicini M et al. (2015) Tumor microenvironment remodeling by 4-methylumbelliferone boosts the antitumor effect of combined immunotherapy in murine colorectal carcinoma. Mol Ther 23(9):1444–1455.

Han X et al. (2015) CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation. Sci Adv 1(7):e1500454

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For Research Use Only. Not for use in diagnostic procedures.