TC-FlAsH™ II In-Cell Tetracysteine Tag Detection Kit contains an expression tag-based fluorescence labeling reagent for live-cell labeling. Mammalian cell lines expressing a protein fused to a tetracysteine tag (CCPGCC) can be labeled with the green-fluorescent FlAsH-EDT2 reagent contained in the kit. The tagged protein is fluorescent only when the labeling reagent is added. BAL wash buffer replaces the previously supplied Disperse Blue 3 and EDT-based wash buffer as an olfactorily more agreeable reagent that yields superior signal to noise.
The kit contains FlAsH-EDT2 labeling reagent (store at -20°C, protected from light) and BAL wash buffer (stored at 4°C). When stored as directed, the kit is stable for 6 months.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product Type
Tetracysteine Tag Detection Kit
Detection Method
Fluorescent
For Use With (Equipment)
Fluorescence Microscope
Assay
Tetracysteine Tag Detection
Color
Green
Label or Dye
FlAsH
Shipping Condition
Wet Ice
Format
Kit
Product Line
TC-FlAsH II
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.