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Gene Editing Basics Learning Center |
Tools and resources to support TALEN and CRISPR applications
Genome editing has emerged as one of humanity’s most powerful technologies in the pursuit of a greater understanding of human health and disease. Less expensive and relatively easy to design, the next generation of gene editing tools is now allowing researchers to target almost any gene for modification in a highly specific and reliable way.
In this educational web hub, you will find a variety of tools and resources to fully support your understanding of gene editing techniques and methods. Learn all you need to know in order to overcome existing hurdles and design successful gene editing experiments, whether for TALEN or CRISPR applications.
Explore free downloads, watch how-to tutorials, gain insights from expert interviews and scientific articles, watch gene editing videos and webinars—and more.
Learn more about gene editing techniques—download gene editing handbook for TALEN and CRISPR basics, CRISPR infographics, and more
Handbook
Gene editing resource guide
Step-by-step guide to help eliminate the trial-and-error of gene editing, allowing you to get answers fast and with little effort.
Application eBook
Key considerations in genome editing
Explore our innovative suite of CRISPR-based editing tools designed to support both basic and applied research applications.
Infographic
CRISPR Tips & Tricks
This infographic will serve as your guide with best practices, pro tips and troubleshooting advice for CRISPR-Cas9.
Infographic
Cas9 Plasmids vs Proteins
Discover how your CRISPR gene editing experiments can benefit from Cas9 proteins.
Infographic
CRISPR for Therapeutics
Discover how CRISPR-Cas9 is driving a new generation of cell engineering for cell-based therapies and key considerations for selecting Cas9 reagents and suppliers.
Blog article
Tips for optimizing sgRNA design
Understand the importance of optimizing sgRNA design and discover related tips and tools.
Watch TALEN and CRISPR Cas9 tutorials—Gene editing methods, step-by-step CRISPR training and more
Training module
Gene editing in pluripotent stem cells
A complete guide for gene editing in PSCs, including all the tools and protocols needed to design, deliver, and screen for preferred gene edits.
Video
A step-by-step workflow for a knock-out experiment in an adherent cell line
In this tutorial video, we demonstrate the process of conducting a HPRT gene knock-out experiment in a HEK293 adherent cell line.
Video
A step-by-step workflow for a knock-in experiment in an adherent cell line
In this tutorial video, we demonstrate the process of conducting a beta actin gene knock-in in a HEK293 adherent cell line.
Video
A step-by-step workflow for a knock-out experiment in iPSCs
In this tutorial video, we demonstrate the process of conducting a gene knock-out experiment in induced pluripotent stem cells.
Video
A step-by-step workflow for a knock-out experiment in T cells
In this tutorial video, we demonstrate the process of conducting a PDCD1 gene knock-out in T cells.
Get insights on gene editing techniques from industry leaders
Video
CRISPR-Cas9: A transformative tool for treating debilitating genetic disease
Dr. Evangelos Kiskinis, Assistant Professor of Neurology and Neuroscience at Northwestern University
Video
How CRISPR provides a unique opportunity to generate rare disease avatars
Dr. Sergio Casas-Tintó, Head of Drosophila Models, Human Disease Unit, Institute for Rare Diseases Research (IIER)
Article
Revolutionizing drug discovery: The role of CRISPR gene editing in target validation
Dr. Panos Zalmas, Head of the Open Targets Validation Lab, Wellcome Sanger Institute
Article
CRISPR-generated CAR-T cells: A new frontier in the treatment of solid tumors
Dr. Krishanu Saha, Associate Professor at the University of Wisconsin-Madison
Article
Collaboration and CRISPR tools move scientists closer to a cure for sight loss
Dr. Yu Holly Chen, Assistant Professor at the University of Alabama, and Dr. Rinki Ratnapriya, Assistant Professor at the Baylor College of Medicine
Article
Modeling rare diseases using CRISPR gene editing—Could fruit flies save lives?
Dr. Sergio Casas-Tintó, Head of Drosophila Models, Human Disease Unit, Institute for Rare Diseases Research (IIER)
Article
Looking to overcome challenges in your gene editing workflow?
Dr. Jason Potter, R&D Director at Thermo Fisher Scientific
Article
Design your CRISPR gene editing experiments in as little as five minutes
Robert Horton, Senior Product Manager at Thermo Fisher Scientific
Watch TALEN and CRISPR webinars
Webinar
The revolution of CRISPR genome editing: An expert panel discussion
Featuring Grazia Iannello, José Inacio, Pia Johansson and Kailash Mangalhara
Webinar
CRISPR gene editing of human primary NK and T cells for cancer immunotherapy
Meisam Naeimi Kararoudi, Director of CRISPR/Gene Editing Core at Ohio State University
Webinar
Conservation genome engineering: A return to the past with technology of the future
Justin Quinn, Director of Product Research at Colossal Biosciences
Webinar
Gene editing in stem cells—Methods and considerations
Hemanta Sarmah, Postdoctoral Research Scholar at Columbia Stem Cell Initiative
Webinar
Considerations to limit off-target edits in your gene editing experiments
Kevin Pridham, Field Application Scientist at Thermo Fisher Scientific
Webinar
The development of GMP-manufactured Cas9 protein for therapeutic applications
Pandhita Pochanard, Product Manager at Thermo Fisher Scientific
Webinar
The growing concern of off-target effects: How to measure and minimize off-target effects in your genome editing experiments
Pandhita Pochanard, Product Manager at Thermo Fisher Scientific
Webinar
Simplified online tools for CRISPR-Cas9 gene editing design and confirmation
Thermo Fisher Scientific
Webinar
Tools and methods for CRISPR-Cas9 screening for functional genomics and drug discovery
Thermo Fisher Scientific
Featured gene editing videos
A Brief History of CRISPR - Part OneWatch this narration by Yasmin Dickinson, University of Oxford, to learn more about the history of CRISPR and gene editing.
A Brief History of CRISPR - Part TwoWatch this narration by Yasmin Dickinson, University of Oxford, to learn more about the history of CRISPR and gene editing.
LABTalks: CRISPR/Cas9: Simple & versatile genome editing toolDr. Namritha Ravinder discusses the powerful new CRISPR/ Cas9 technology for genome editing as part of our LABTalks series.
The Power of CRISPR Genome EditingIt’s your time to change the world. And it’s our promise to empower you with cell engineering tools and solutions that help you get results even faster. Increasing your chances of success at every step.
Next level CRISPR editing—Cas9 RNPCas9 protein and gRNA form a very stable RNP complex that provides the next level of cleavage efficiency over CRISPR-Cas9 vector and mRNA-based systems when paired with Invitrogen™ Lipofectamine™ CRISPRMAX™ Cas 9 Transfection Reagent.
CRISPR-Cas9 editing made easyWe offer our state-of-the-art online Invitrogen™ CRISPR Search and Design Tool along with Invitrogen™ CRISPR-Cas9 editing products in four formats: an all-in-one expression vector, Cas9 mRNA, Cas9 protein, and CRISPR library services.
Genome editing with GeneArt Precision TALsNew and powerful way to edit genomes and control gene function based on a talk about the TAL effector proteins that were first identified as bacterial plant pathogens.
Featured products to support gene editing methods and applications
For Research Use Only. Not for use in diagnostic procedures.