Pluripotent Stem Cell (PSC) Education | Thermo Fisher Scientific - US

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Your journey to stem cell excellence begins with Gibco PSC Education. From textbook concepts to successful practices, this top-tier educational program offers a wide variety of tools and resources to enable your pluripotent stem cell research success.

Explore the modules below to access free-to-use handbooks, virtual trainings, webinars, and the expertise you need to gain confidence in the lab.

Handbooks

Download a copy of the
PSC Resource Handbook

Download a copy of the
PSC Protocol Handbook

Download a copy of the
Neurobiology Protocol Handbook

Download a copy of the
Research Services Handbook

Download a copy of the
Cell Biology Handbook

Virtual training

Thawing pluripotent stem cells

Training module: Thawing pluripotent stem cells

A detailed guide through our protocol for thawing induced pluripotent stem cells (iPSCs) using StemFlex Medium. Access course

Gene editing in pluripotent stem cells

Training module: Gene editing in pluripotent stem cells

A complete guide for gene editing in PSCs, including all the tools and protocols needed to design, deliver, and screen for preferred gene edits in PSCs.
Access course

StemScale PSC Suspension Medium

Training module: StemScale PSC Suspension Medium—Innovation in stem cell suspension culture

Preparation and maintenance of PSCs in suspension culture using the new Gibco StemScale PSC Suspension Medium. Access course

Neural organoid generation from pluripotent stem cells

Training module: Neural organoid generation from pluripotent stem cells

Learn how to develop neural organoids from PSCs following the steps of our application note.

Access course

Getting started in 3D cell culture

Training module: Getting started in 3D cell culture

Get started with 3D cell culture models by exploring 3D cell culture background, model requirements, creation methods, and development considerations. Access course

Generation of Human Induced Pluripotent Stem Cells

Training module: Generation of Human Induced Pluripotent Stem Cells(hiPSCs)

A complete guide for reprogramming somatic cells into pluripotent stem cells (PSCs) using the CytoTune 2.0 Sendai Reprogramming Kit.
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Cardiomyocyte Differentiation

Training module: Cardiomyocyte Differentiation

An easy-to-use guide for the preparation and differentiation of human pluripotent stem cells into functional cardiomyocytes for basic and translational research.
Access course

How-to videos

How to culture pluripotent stem cells in suspension: Initiation of PSC cultures in suspension using StemScale PSC Suspension Medium

Learn how to Initiate pluripotent stem cells in suspension using StemScale PSC Suspension Medium, a scalable and esay-to-use medium for PSC expansion.

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Up NextHow to culture pluripotent stem cells in suspension: Initiation of PSC cultures in suspension using StemScale PSC Suspension Medium
Learn how to Initiate pluripotent stem cells in suspension using StemScale PSC Suspension Medium, a scalable and esay-to-use medium for PSC expansion.
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Up NextHow to prepare StemFlex Medium
This video will provide step-by-step guidance on how to prepare complete Gibco™ StemFlex™ Medium for the feeder-free culture of pluripotent stem cells.
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Up NextHow to culture pluripotent stem cells in suspension: Maintenance and Feeding of PSC cultures in suspension using StemScale PSC Suspension Medium
Learn step-by-step how to maintain and feed pluripotent stem cells in suspension using StemScale™ PSC Suspension Medium, a new dimension to PSC culture.
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Up NextHow to culture pluripotent stem cells in suspension: Passaging of PSC cultures in suspension using StemScale PSC Suspension Medium
Learn step-by-step how to passage human pluripotent stem cells in suspension using StemScale™ PSC Suspension Medium, a new dimension to PSC culture.
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Up NextHow to prepare Essential 8 Medium for pluripotent stem cell culture
This video will show you how to prepare complete Essential 8 Medium for the consistent, robust, cost-effective feeder-free culture of PSCs.
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Up NextHow to adapt pluripotent stem cells from other PSC media into Essential 8 Medium
This video will show you how to adapt PSCs adapted to feeder-based or feeder-free media to Essential 8 Medium and Vitronectin.
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Up NextPassaging reagents for PSC culture
This video provides an overview of different passaging reagents which can be used to accomplish varying degrees of dissociation of pluripotent stem cells.
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Up NextHow to passage pluripotent stem cells in Essential 8 Medium
This video outlines simple passaging for feeder-free pluripotent stem cell culture with Essential 8™ Medium
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Up NextSelecting a matrix for PSC culture
This video will help you select a matrix to ensure success when culturing your pluripotent stem cells.
3:13
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Up NextHow to prepare vitronectin substrate plates for pluripotent stem cell culture
This video will show you how to coat culture plates with vitronectin, so you will be ready to transfer newly forming iPSC colonies or frozen cultures to Essential 8 Medium.
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Up NextHow to cryopreserve pluripotent stem cells
This video will provide step-by-step guidance on how to cryopreserve pluripotent stem cells (PSCs).
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Up NextHow to differentiate pluripotent stem cells (PSC) into cardiomyocytes
This video provides an overview of the protocols used for differentiating human pluripotent stem cells in to beating cardiomyocytes using the Gibco PSC Cardiomyocyte Differentiation Kit. See how to get beating cardiomyocytes in three simple steps.
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Up NextHow to induce pluripotent stem cells in to definitive endoderm
This video provides an overview of the protocols used for inducting human pluripotent stem cells in to definitive endoderm in just 48 hours using the Gibco PSC Definitive Endoderm Induction Kit. See how to get definitive endoderm cells in just 2 days
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Up NextHow to use Gibco PSC Neural Induction Medium
This video will show you how Gibco® PSC Neural Induction Medium generates up to 20 million neural stem cells from pluripotent stem cells in 7 days.
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Up NextHow to Use the TaqMan hPSC Scorecard Panel
This video will show you how to prepare and run the TaqMan hPSC Scorecard Panel plates.
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See why researchers value PSC Education

"The virtual environment continues to improve each year with outstanding resources."

— 24 Hours of Stem Cells attendee, Researcher, Australia

"I have now been through the PSC Handbook, and it is an amazing piece of resource. I found it very useful because I am currently planning for future experiments, and I have chosen a few products that I would like to try out. I suspect they work well, which means I will definitely be ordering more in the future."

— PSC Handbook viewer, Researcher, Europe

"It was a great course, which provided me with useful information and more importantly, the 'hands-on' practical time that will prove vital in spotting good colonies! I would not hesitate in recommending the course to others in the industry."

— Gibco PSC Workshop attendee, Researcher, USA

"I'm a visual learner so the virtual environment was very beneficial. It felt like I was actually in the lab going through the motions. Great to see such a realistic approach to research!"

— PSC Culture Virtual Lab participant, Researcher, USA

"This is the best workshop! I would recommend everybody new to this field and trying to starting iPSC work attend this workshop."

— Gibco PSC Workshop attendee, Researcher, UK

"A really cool, intriguing spin on training with lots of great information."

— PSC Culture Virtual Lab participant, Researcher, USA

Other tools & resources

For Research Use Only. Not for use in diagnostic procedures.

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