| SARS-CoV-2 Neutralizing Antibody Responses towards Full-Length Spike Protein and the Receptor-Binding Domain | The Journal of Immunology | 2021 | COVID | Expi293 |
| Selective activation of pro-anti-IL-1β antibody enhances specificity for autoinflammatory disorder therapy | Scientific Reports | 2021 | Immunotherapy | Expi293 |
| Isolation and characterization of porcine monoclonal antibodies revealed two distinct serotype-independent epitopes on VP2 of foot-and-mouth disease virus | Journal of General Virology | 2021 | Infectious Disease | ExpiCHO |
| Bi-specific and Tri-specific NK Cell Engagers: The New Avenue of Targeted NK Cell Immunotherapy | Molecular Diagnosis & Therapy | 2021 | Immunotherapy | Expi293 |
| Attenuating nicotine’s effects with high affinity human anti-nicotine monoclonal antibodies | PLoS One | 2021 | Immunology | ExpiCHO |
| Cryo-EM structures of inhibitory antibodies complexed with arginase 1 provide insight into mechanism of action | Communications Biology | 2021 | Protein Structure/Function | ExpiCHO |
| Screening of potent neutralizing antibodies against SARS-CoV-2 using convalescent patients-derived phage-display libraries | Cell Discovery | 2021 | COVID | Expi293 |
| Solenoid architecture of HUWE1 contributes to ligase activity and substrate recognition | Molecular Cell | 2021 | Protein Structure/Function | Expi293 |
| Characterization of the SARS-CoV-2 S Protein: Biophysical, Biochemical, Structural, and Antigenic Analysis | ACS Omega | 2020 | COVID | Expi293 and ExpiCHO |
| Production of SARS-CoV-2 virus-like particles in insect cells | MDPI Vaccines | 2021 | COVID | ExpiSf |
| Evaluation of novel recombinant porcine circovirus type 2d (PCV2d) vaccine in pigs naturally infected with PCV2d | Vaccine | 2021 | Vaccine | ExpiSf |
| Enhancement of antibody-dependent cellular cytotoxicity and phagocytosis in anti‐HIV‐1 bovine chimeric broadly-neutralizing antibodies | Journal of Virology | 2021 | Infectious Diseases | Expi293 |
| Proprotein Convertase Is the Highest-Level Activator of the Alternative Complement Pathway in the Blood | The Journal of Immunology | 2021 | Protein Structure/Function | ExpiCHO |
| Development of a highly specific serodiagnostic ELISA for West Nile virus infection using subviral particles | Scientific Reports | 2021 | Biotechnology | Expi293 |
| Meningeal lymphatics affect microglia responses and anti-Aβ immunotherapy | Nature | 2021 | Immunotherapy | Expi293 |
| Structures of Foot-and-mouth Disease Virus with neutralizing antibodies derived from recovered natural host reveal a mechanism for cross-serotype neutralization | PLOS Pathogens | 2021 | Virus Structure/Function | ExpiCHO |
| Broadly neutralizing antibody cocktails targeting Nipah virus and Hendra virus fusion glycoproteins | Nature Structural & Molecular Biology | 2021 | Infectious Diseases | ExpiCHO |
| Advanced glycation end products (AGEs) synergistically potentiated the proinflammatory action of lipopolysaccharide (LPS) and high mobility group box-1 (HMGB1) through their direct interactions | Molecular Biology Reports | 2020 | Protein Structure/Function | ExpiSf |
| Broad and potently neutralizing monoclonal antibodies isolated from human survivors of New World hantavirus infection | Cell Reports | 2021 | Infectious Diseases | Expi293 and ExpiCHO |
| Antibody evasion by the P.1 strain of SARS-CoV-2 | Cell | 2021 | COVID | Expi293 and ExpiCHO |
| Long-term persistence of RBD + memory B cells encoding neutralizing antibodies in SARS-CoV-2 infection | Cell Reports Medicine | 2021 | COVID | Expi293 |
| Immunogenicity of prime-boost protein subunit vaccine strategies against SARS-CoV-2 in mice and macaques | Nature Communications | 2021 | COVID | Expi293 |
| Conserved and Novel Mouse CD8 T Cell Epitopes within SARS-CoV-2 Spike Receptor Binding Domain Protein Identified following Subunit Vaccination | The Journal of Immunology | 2021 | COVID | Expi293 |
| Neutralizing antibody responses to SARS-CoV-2 in symptomatic COVID-19 is persistent and critical for survival | Nature Communications | 2021 | COVID | Expi293 |
| Heparin-binding VEGFR1 variants as long-acting VEGF inhibitors for treatment of intraocular neovascular disorders | PNAS | 2021 | Protein Structure/Function | Expi293 |
| Implementing a method for engineering multivalency to substantially enhance binding of clinical trial anti-SARS-CoV-2 antibodies to wildtype spike and variants of concern proteins | Scientific Reports | 2021 | COVID | Expi293 |
| Mining of linear B cell epitopes of SARS-CoV-2 ORF8 protein from COVID-19 patients | Emerging Microbes & Infections | 2021 | COVID | Expi293 |
| A bispecific antibody agonist of the IL-2 heterodimeric receptor preferentially promotes in vivo expansion of CD8 and NK cells | Scientific Reports | 2021 | Immunology | ExpiCHO |
| Antigen-driven autoantibody production in lungs of interstitial lung disease with autoimmune disease | Journal of Autoimmunity | 2021 | Immunology | Expi293 |
| Engineering a natural ligand-based CAR: directed evolution of the stress-receptor NKp30 | Cancer Immunology, Immunotherapy | 2021 | Immunotherapy | Expi293 |
| Protective neutralizing antibodies from human survivors of Crimean-Congo hemorrhagic fever | Cell | 2021 | Infectious Diseases | ExpiCHO |
| Attenuating CD3 affinity in a PSMAxCD3 bispecific antibody enables killing of prostate tumor cells with reduced cytokine release | Journal for ImmunoTherapy of Cancer | 2021 | Immunotherapy | ExpiCHO |
| Performance of a SARS CoV-2 antibody ELISA based on simultaneous measurement of antibodies against the viral nucleoprotein and receptor-binding domain | European Journal of Clinical Microbiology & Infectious Diseases | 2021 | COVID | Expi293 |
| A novel multiplexed Enzyme-Linked Immunosorbent Assay for the detection of IgG seroreactivity to cytomegalovirus (CMV) UL144 | Journal of Clinical Microbiology | 2021 | Biotechnology | Expi293 |
| Polymorphic SERPINA3-R124C reduces pathogenesis of its wild type by shortening the life time of oligomeric Aβ | Bioscience, Biotechnology, and Biochemistry | 2021 | Protein Structure/Function | Expi293 |
| Development of CpG-adjuvanted stable prefusion SARS-CoV-2 spike antigen as a subunit vaccine against COVID-19 | Scientific Reports | 2020 | COVID | ExpiCHO |
| Nasal delivery of an IgM offers broad protection from SARS-CoV-2 variants | Nature | 2021 | COVID | Expi293 and ExpiCHO |
| Multivalency transforms SARS-CoV-2 antibodies into ultrapotent neutralizers | Nature Communications | 2021 | COVID | Expi293 |
| Conformation-sensitive targeting of lipid nanoparticles for RNA therapeutics | Nature Nanotechnology | 2021 | Biotechnology | Expi293 |
| Primary, Recall, and Decay Kinetics of SARS-CoV-2 Vaccine Antibody Responses | ACS Nano | 2021 | COVID | Expi293 |
| Generation of a safe and efficacious llama single-domain antibody fragment (vHH) targeting the membrane-proximal region of 4-1BB for engineering therapeutic bispecific antibodies for cancer | Journal for ImmunoTherapy of Cancer | 2021 | Immunotherapy | ExpiCHO |
| High-affinity five/six-letter DNA aptamers with superior specificity enabling the detection of dengue NS1 protein variants beyond the serotype identification | Nucleic Acids Research | 2021 | Infectious Diseases | ExpiCHO |
| A Rapid Assay for SARS-CoV-2 Neutralizing Antibodies That Is Insensitive to Antiretroviral Drugs | The Journal of Immunology | 2021 | COVID | Expi293 |
| Structural mechanism of laminin recognition by integrin | Nature Communications | 2021 | Protein Structure/Function | Expi293 |
| Ultrapotent antibodies against diverse and highly transmissible SARS-CoV-2 variants | Science | 2021 | COVID | Expi293 |
| Phosphatidylserine-Targeting Monoclonal Antibodies Exhibit Distinct Biochemical and Cellular Effects on Anti-CD3/CD28–Stimulated T Cell IFN-γ and TNF-α Production | The Journal of Immunology | 2021 | Immunology | Expi293 |
| Novel anti-4-1BB×PD-L1 bispecific antibody augments anti-tumor immunity through tumor-directed T-cell activation and checkpoint blockade | Journal for ImmunoTherapy of Cancer | 2021 | Immunotherapy | ExpiCHO |
| Diverse high-affinity DNA aptamers for wild-type and B.1.1.7 SARS-CoV-2 spike proteins from a pre-structured DNA library | Nucleic Acids Research | 2021 | COVID | Expi293 |
| Broadly cross-reactive human antibodies that inhibit genogroup I and II noroviruses | Nature Communications | 2021 | Infectious Diseases | ExpiCHO |
| Simultaneous evaluation of antibodies that inhibit SARS-CoV-2 variants via multiplex assay | JCI Insight | 2021 | COVID | Expi293 |
| CpG-adjuvanted stable prefusion SARS-CoV-2 spike protein protected hamsters from SARS-CoV-2 challenge | Scientific Reports | 2021 | COVID | ExpiCHO |
| Site-Specific Bioconjugation Using SMARTag® Technology: A Practical and Effective Chemoenzymatic Approach to Generate Antibody–Drug Conjugates | Methods in Molecular Biology | 2019 | Biotechnology | ExpiCHO |
| Optimizing high throughput antibody purification by using continuous chromatography media | Protein Expression and Purification | 2019 | Biotechnology | Expi293 and ExpiCHO |
| Simply Mixing Poly Protein G with Detection Antibodies Enhances the Detection Limit and Sensitivity of Immunoassays | Analytical Chemistry | 2019 | Biotechnology | Expi293 |
| Site Selective Antibody-Oligonucleotide Conjugation via Microbial Transglutaminase | Molecules | 2019 | Biotechnology | ExpiCHO |
| Rapid isolation and enrichment of extracellular vesicle preparations using anion exchange chromatography | Nature Scientific Reports | 2018 | Biotechnology | Expi293 |
| Transient CHO expression platform for robust antibody production and its enhanced N‐glycan sialylation on therapeutic glycoproteins | Biotechnology Progress | 2018 | Biotechnology | Expi293 and ExpiCHO |
| Scalable method utilizing low pH for DNA removal in the harvest of recombinant adeno-associated virus vectors | Journal of Chromatography B | 2019 | Gene Therapy | Expi293 |
| Sodium Chloride Enhances Recombinant Adeno-Associated Virus Production in a Serum-Free Suspension Manufacturing Platform Using the Herpes Simplex Virus System | Human Gene Therapy Methods | 2017 | Gene Therapy | Expi293 |
| Chemically Defined, High-Density Insect Cell-Based Expression System for Scalable AAV Vector Production | Molecular Therapy Methods and Clinical Development | 2020 | Gene Therapy | ExpiSf |
| Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally Distinct Vectors | Molecular Therapy Methods and Clinical Development | 2020 | Gene Therapy | Expi293 |
| Gene delivery of a modified antibody to Aβ reduces progression of murine Alzheimer’s disease | PLoS One | 2019 | Immunotherapy | Expi293 |
| Vectored delivery of anti-SIV envelope targeting mAb via AAV8 protects rhesus macaques from repeated limiting dose intrarectal swarm SIVsmE660 challenge | PLoS Pathogens | 2018 | Immunotherapy | Expi293 |
| Establishment of P38Bf, a Core-Fucose-Deficient Mouse-Canine Chimeric Antibody Against Dog Podoplanin | Monoclonal Antibodies in Immunodiagnosis and Immunotherapy | 2018 | Immunotherapy | ExpiCHO |
| Enhancing protective microglial activities with a dual function TREM2 antibody to the stalk region | EMBO Molecular Medicine | 2020 | Immunotherapy | ExpiCHO |
| Specific Sialoforms Required for the Immune Suppressive Activity of Human Soluble CD52 | Frontiers in Immunology | 2019 | Immunotherapy | Expi293 |
| Design and preclinical characterization of ALXN1210: a novel anti-C5 antibody with extended duration of action | PLOS One | 2018 | Immunotherapy | Expi293 |
| Antibody–Drug Conjugates Using Mouse–Canine Chimeric Anti-Dog Podoplanin Antibody Exerts Antitumor Activity in a Mouse Xenograft Model | Monoclonal Antibodies in Immunodiagnosis and Immunotherapy | 2020 | Immunotherapy | ExpiCHO |
| Antibody-Based Targeting of Cell Surface GRP94 Specifically Inhibits Cetuximab-Resistant Colorectal Cancer Growth | Biomolecules | 2019 | Immunotherapy | Expi293 |
| Blocking Monocytic Myeloid-Derived Suppressor Cell Function via Anti-DC-HIL/GPNMB Antibody Restores the In Vitro Integrity of T Cells from Cancer Patients | Clinical Cancer Research | 2019 | Immunotherapy | ExpiCHO |
| Dual targeting of RANKL and PD‐1 with a bispecific antibody improves anti‐tumor immunity | Clinical and Translational Immunology | 2019 | Immunotherapy | Expi293 and ExpiCHO |
| The Strong Propensity of Cadherin‐23 for Aggregation Inhibits Cell Migration | Molecular Oncology | 2019 | Immunotherapy | ExpiCHO |
| Engineering and stable production of recombinant IgE for cancer immunotherapy and AllergoOncology | Journal of Allergy and Clinical Immunology | 2018 | Immunotherapy | Expi293 |
| Differentiated agonistic antibody targeting CD137 eradicates large tumors without hepatotoxicity | Journal of Clinical Investigation | 2020 | Immunotherapy | Expi293 and ExpiCHO |
| Enhanced Production of Anti-PD1 Antibody in CHO Cells through Transient Co-Transfection with Anti-Apoptotic Gene Bcl-xL Combined with Rapamycin | Bioprocess and Biosystems Engineering | 2018 | Immunotherapy | ExpiCHO |
| Discovery and characterization of single-domain antibodies for polymeric Ig receptor-mediated mucosal delivery of biologics | mAbs | 2020 | Immunology | Expi293 and ExpiCHO |
| Engineering a high-affinity peptide binding site into the anti-CEA mAb M5A | Protein Engineering Design & Selection | 2017 | Immunology | ExpiCHO |
| Human IgG subclass cross-species reactivity to mouse and cynomolgus monkey Fcγ receptors | Immunology Letters | 2018 | Immunology | Expi293 |
| Early identification of unusually clustered mutations and root causes in therapeutic antibody development | Biotechnology and Bioengineering | 2018 | Immunology | ExpiCHO |
| Stabilization of the CD81 Large Extracellular Loop with De Novo Disulfide Bonds Improves Its Amenability for Peptide Grafting | MDPI Pharmaceutics | 2018 | Immunology | ExpiCHO |
| Rapid charge variant analysis of monoclonal antibodies to support lead candidate biopharmaceutical development | Journal of Chromatography B | 2018 | Immunology | Expi293 and ExpiCHO |
| Lysosomal Protein Thermal Stability Does Not Correlate With Cellular Half-Life: Global Observations and a Case Study of Tripeptidyl-Peptidase 1 | Biochemical Journal | 2020 | Protein Structure/Function | ExpiCHO |
| Molecular basis for dyneinopathies reveals insight into dynein regulation and dysfunction | eLife Science | 2019 | Protein Structure/Function | ExpiSf |
| Comparative Glycopeptide Analysis for Protein Glycosylation by Liquid Chromatography and Tandem Mass Spectrometry: Variation in Glycosylation Patterns of Site-Directed Mutagenized Glycoprotein | International Journal of Analytical Chemistry | 2018 | Protein Structure/Function | ExpiCHO |
| Mammalian plasma fetuin-B is a selective inhibitor of ovastacin and meprin metalloproteinases | Nature Scientific Reports | 2019 | Protein Structure/Function | ExpiCHO |
| Tailored tetravalent antibodies potently and specifically activate Wnt/Frizzled pathways in cells, organoids and mice | eLife Science | 2019 | Protein Structure/Function | Expi293 |
| IL-2 Shapes the Survival and Plasticity of IL-17–Producing γδ T Cells | Journal of Immunology | 2017 | Protein Structure/Function | Expi293 |
| Identification of the gene that codes for the σ2 receptor | PNAS | 2017 | Protein Structure/Function | Expi293 |
| Novel CH1:CL interfaces that enhance correct light chain pairing in heterodimeric bispecific antibodies | Protein Engineering, Design and Selection | 2017 | Protein Structure/Function | Expi293 and ExpiCHO |
| Mechanically interlocked functionalization of monoclonal antibodies | Nature Communications | 2018 | Protein Structure/Function | ExpiCHO |
| Antibody-drug conjugate library prepared by scanning insertion of the aldehyde tag into IgG1 constant regions | mAbs | 2018 | Protein Structure/Function | ExpiCHO |
| Pac1/LIS1 promotes an uninhibited conformation of dynein that coordinates its localization and activity | Nature Cell Biology | 2020 | Protein Structure/Function | ExpiSf |
| The Biochemical and Functional Characterization of M28 Aminopeptidase Protein Secreted by Acanthamoeba spp. on Host Cell Interaction | Molecules | 2019 | Protein Structure/Function | ExpiCHO |
| Cross-neutralization of SARS-CoV-2 by a human monoclonal SARS-CoV antibody | Nature | 2020 | COVID | ExpiCHO |
| Activation of latent transforming growth factor-β1, a conserved function for pregnancy-specific beta 1-glycoproteins | Molecular Human Reproduction | 2018 | Protein Structure/Function | Expi293 and ExpiCHO |
| Expression of glycosylated human prolactin in HEK293 cells and related N-glycan composition analysis | AMB Express | 2019 | Protein Structure/Function | Expi293 |
| Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays | Protein Expression and Purification | 2020 | COVID | Expi293 |
| Oligomeric state of the ZIKV E protein defines protective immune responses | Nature Communications | 2019 | Vaccine | Expi293 |
| In Vitro Assembly and Stabilization of Dengue and Zika Virus Envelope Protein Homo-Dimers | Nature Scientific Reports | 2017 | Vaccine | Expi293 |
| HIV-1 vaccine design through minimizing envelope metastability | Science Advances | 2018 | Vaccine | ExpiCHO |
| Soluble Recombinant Hemagglutinin Protein of H1N1pdm09 Influenza Virus Elicits Cross-Protection Against a Lethal H5N1 Challenge in Mice | Frontiers in Microbiology | 2019 | Vaccine | Expi293 |
| Nanoparticle delivery of a tetravalent E protein subunit vaccine induces balanced, type-specific neutralizing antibodies to each dengue virus serotype | PLOS Neglected Tropical Diseases | 2018 | Vaccine | Expi293 |
| Synthetic DNA delivery by electroporation promotes robust in vivo sulfation of broadly neutralizing anti-HIV immunoadhesin eCD4-Ig | EBioMedicine | 2018 | Vaccine | Expi293 |
| Neuraminidase Inhibition Contributes to Influenza A Virus Neutralization by Anti-Hemagglutinin Stem Antibodies | Journal of Experimental Medicine | 2019 | Vaccine | Expi293 |
| Zika virus-like particle (VLP) based vaccine | PLOS Neglected Tropical Diseases | 2017 | Vaccine | Expi293 |
| Framework Mutations of the 10-1074 bnAb Increase Conformational Stability, Manufacturability, and Stability While Preserving Full Neutralization Activity | Journal of Pharmaceutical Sciences | 2019 | Vaccine | ExpiCHO |
| Recurring and Adaptable Binding Motifs in Broadly Neutralizing Antibodies to Influenza Virus Are Encoded on the D3-9 Segment of the Ig Gene | Cell Host & Microbe | 2018 | Vaccine | ExpiCHO |
| Broadly neutralizing human antibodies against dengue virus identified by single B cell transcriptomics | eLife Science | 2019 | Vaccine | Expi293 and ExpiCHO |
| Immunoglobulin fragment F(ab’)2 against RBD potently neutralizes SARS-CoV-2 in vitro | Antiviral Research | 2020 | COVID | ExpiCHO |
| Development of an inactivated vaccine candidate for SARS-CoV-2 | Science | 2020 | COVID | Expi293 |
| Optimization of Surface Display of DENV2 E Protein on a Nanoparticle to Induce Virus Specific Neutralizing Antibody Responses | Bioconjugate Chemistry | 2018 | Vaccine | Expi293 |
| Proof of concept for rational design of hepatitis C virus E2 core nanoparticle vaccines | Science Advances | 2020 | Vaccine | ExpiCHO |
| Preventing an Antigenically Disruptive Mutation in Egg-Based H3N2 Seasonal Influenza Vaccines by Mutational Incompatibility | Cell Host & Microbe | 2019 | Vaccine | ExpiCHO |
| Crystal structures of human Fabs targeting the Bexsero meningococcal vaccine antigen NHBA | Acta Crystallographica Section F Structural Biology Communications | 2017 | Vaccine | Expi293 |
| SARS-CoV-2 Seroconversion in Humans: A Detailed Protocol for a Serological Assay, Antigen Production, and Test Setup | Current Protocols in Microbiology | 2020 | COVID | Expi293 |
| Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation | Science | 2020 | COVID | Expi293 |
| A highly conserved cryptic epitope in the receptor-binding domains of SARS-CoV-2 and SARS-CoV | Science | 2020 | COVID | Expi293 and ExpiCHO |
| Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigen | PNAS | 2017 | COVID | Expi293 |
| Structural Basis for Potent Neutralization of Betacoronaviruses by Single-domain Camelid Antibodies | Cell | 2020 | COVID | ExpiCHO |
| Cryo-EM structure of full-length HIV-1 Env bound with the Fab of antibody PG16 | Journal of Molecular Biology | 2020 | COVID | Expi293 |
| Structure-based design of prefusion-stabilized SARS-CoV-2 spikes | Science | 2020 | COVID | ExpiCHO |
| Potent neutralizing antibodies directed to multiple epitopes on SARS-CoV-2 spike | Nature | 2020 | COVID | Expi293 |
How to set up your lab for mammalian transient protein expression
ExpiCHO: Transient CHO Expression Protocol
Expi293: Transient HEK 293 Expression Protocol